2r9a: Difference between revisions
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'''Crystal structure of human XLF''' | '''Crystal structure of human XLF''' | ||
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[[Category: Andres, S N.]] | [[Category: Andres, S N.]] | ||
[[Category: Junop, M S.]] | [[Category: Junop, M S.]] | ||
[[Category: | [[Category: Alternative splicing]] | ||
[[Category: | [[Category: Cernunnos,non-homologous end joining]] | ||
[[Category: | [[Category: Disease mutation]] | ||
[[Category: | [[Category: Dna damage]] | ||
[[Category: | [[Category: Dna double strand break repair]] | ||
[[Category: | [[Category: Dna repair]] | ||
[[Category: | [[Category: Nucleus]] | ||
[[Category: | [[Category: Protein binding]] | ||
[[Category: | [[Category: Xlf]] | ||
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Revision as of 13:27, 4 May 2008
Crystal structure of human XLF
Overview
DNA double-strand breaks represent one of the most severe forms of DNA damage in mammalian cells. One pathway for repairing these breaks occurs via nonhomologous end-joining (NHEJ) and depends on XRCC4, LigaseIV, and Cernunnos, also called XLF. Although XLF stimulates XRCC4/LigaseIV to ligate mismatched and noncohesive DNA ends, the mechanistic basis for this function remains unclear. Here we report the structure of a partially functional 224 residue N-terminal fragment of human XLF. Despite only weak sequence similarity, XLF(1-170) shares structural homology with XRCC4(1-159). However, unlike the highly extended 130 A helical domain observed in XRCC4, XLF adopts a more compact, folded helical C-terminal region involving two turns and a twist, wrapping back to the structurally conserved N terminus. Mutational analysis of XLF and XRCC4 reveals a potential interaction interface, suggesting a mechanism for how XLF stimulates the ligation of mismatched ends.
About this Structure
2R9A is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human XLF: a twist in nonhomologous DNA end-joining., Andres SN, Modesti M, Tsai CJ, Chu G, Junop MS, Mol Cell. 2007 Dec 28;28(6):1093-101. PMID:18158905 Page seeded by OCA on Sun May 4 16:27:24 2008
