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[[Image:3boy.jpg|left|200px]]
[[Image:3boy.jpg|left|200px]]


{{Structure
<!--
|PDB= 3boy |SIZE=350|CAPTION= <scene name='initialview01'>3boy</scene>, resolution 1.70&Aring;
The line below this paragraph, containing "STRUCTURE_3boy", creates the "Structure Box" on the page.
|SITE=
You may change the PDB parameter (which sets the PDB file loaded into the applet)
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY=
or leave the SCENE parameter empty for the default display.
|GENE= hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
-->
|DOMAIN=
{{STRUCTURE_3boy|  PDB=3boy |  SCENE= }}  
|RELATEDENTRY=[[1vea|1VEA]], [[1wpt|1WPT]], [[1wmq|1WMQ]], [[1wpu|1WPU]], [[1wrq|1WRQ]], [[1wpv|1WPV]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3boy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3boy OCA], [http://www.ebi.ac.uk/pdbsum/3boy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3boy RCSB]</span>
}}


'''Crystal structure of the HutP antitermination complex bound to the HUT mRNA'''
'''Crystal structure of the HutP antitermination complex bound to the HUT mRNA'''
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==About this Structure==
==About this Structure==
3BOY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry 2GZT. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOY OCA].  
3BOY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2gzt 2gzt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOY OCA].  


==Reference==
==Reference==
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[[Category: Shinkai, A.]]
[[Category: Shinkai, A.]]
[[Category: Yokoyama, S.]]
[[Category: Yokoyama, S.]]
[[Category: activator]]
[[Category: Activator]]
[[Category: anti-termination]]
[[Category: Anti-termination]]
[[Category: histidine metabolism]]
[[Category: Histidine metabolism]]
[[Category: hutp]]
[[Category: Hutp]]
[[Category: hutp-rna complex]]
[[Category: Hutp-rna complex]]
[[Category: national project on protein structural and functional analyse]]
[[Category: National project on protein structural and functional analyse]]
[[Category: nppsfa]]
[[Category: Nppsfa]]
[[Category: riken structural genomics/proteomics initiative]]
[[Category: Riken structural genomics/proteomics initiative]]
[[Category: rna-binding]]
[[Category: Rna-binding]]
[[Category: rsgi]]
[[Category: Rsgi]]
[[Category: structural genomic]]
[[Category: Structural genomic]]
[[Category: transcription regulation]]
[[Category: Transcription regulation]]
[[Category: transcription/rna complex]]
[[Category: Transcription/rna complex]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:58:12 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:27:58 2008''

Revision as of 17:58, 4 May 2008

File:3boy.jpg

Template:STRUCTURE 3boy

Crystal structure of the HutP antitermination complex bound to the HUT mRNA


Overview

HutP is an L-histidine-activated RNA binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis by binding to cis-acting regulatory sequences on the hut mRNA. The crystal structure of HutP complexed with an L-histidine analog showed a novel fold; there are four antiparallel beta strands in the central region of each monomer, with two alpha helices each on the front and back. Two HutP monomers form a dimer, and three dimers are arranged in crystallographic 3-fold symmetry to form a hexamer. A histidine analog was located in between the two monomers of HutP, with the imidazole group of L-histidine hydrogen bonded to Glu81. An activation mechanism is proposed based on the identification of key residues of HutP. The HutP binding region in hut mRNA was defined: it consists of three UAG trinucleotide motifs separated by four spacer nucleotides. Residues of HutP potentially important for RNA binding were identified.

About this Structure

3BOY is a Protein complex structure of sequences from Bacillus subtilis. This structure supersedes the now removed PDB entry 2gzt. Full crystallographic information is available from OCA.

Reference

Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:15242603 Page seeded by OCA on Sun May 4 20:58:12 2008

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