3psg: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:3psg.gif|left|200px]] | [[Image:3psg.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_3psg", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
| | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | ||
| | or leave the SCENE parameter empty for the default display. | ||
--> | |||
{{STRUCTURE_3psg| PDB=3psg | SCENE= }} | |||
}} | |||
'''THE HIGH RESOLUTION CRYSTAL STRUCTURE OF PORCINE PEPSINOGEN''' | '''THE HIGH RESOLUTION CRYSTAL STRUCTURE OF PORCINE PEPSINOGEN''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
3PSG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry | 3PSG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1psg 1psg]. The following page contains interesting information on the relation of 3PSG with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb12_1.html Pepsin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSG OCA]. | ||
==Reference== | ==Reference== | ||
| Line 29: | Line 26: | ||
[[Category: Koelsch, G.]] | [[Category: Koelsch, G.]] | ||
[[Category: Remington, S J.]] | [[Category: Remington, S J.]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:11:10 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 19:11, 4 May 2008
THE HIGH RESOLUTION CRYSTAL STRUCTURE OF PORCINE PEPSINOGEN
Overview
The structure of porcine pepsinogen at pH 6.1 has been refined to an R-factor of 0.173 for data extending to 1.65 A. The final model contains 180 solvent molecules and lacks density for residues 157-161. The structure of this aspartic proteinase zymogen possesses many of the characteristics of pepsin, the mature enzyme. The secondary structure of the zymogen consists predominantly of beta-sheet, with an approximate 2-fold axis of symmetry. The activation peptide packs into the active site cleft, and the N-terminus (1P-9P) occupies the position of the mature N-terminus (1-9). Thus changes upon activation include excision of the activation peptide and proper relocation of the mature N-terminus. The activation peptide or residues of the displaced mature N-terminus make specific interactions with the substrate binding subsites. The active site of pepsinogen is intact; thus the lack of activity of pepsinogen is not due to a deformation of the active site. Nine ion pairs in pepsinogen may be important in the advent of activation and involve the activation peptide or regions of the mature N-terminus which are relocated in the mature enzyme. The activation peptide-pepsin junction, 44P-1, is characterized by high thermal parameters and weak density, indicating a flexible structure which would be accessible to cleavage. Pepsinogen is an appropriate model for the structures of other zymogens in the aspartic proteinase family.
About this Structure
3PSG is a Single protein structure of sequence from Sus scrofa. This structure supersedes the now removed PDB entry 1psg. The following page contains interesting information on the relation of 3PSG with [Pepsin]. Full crystallographic information is available from OCA.
Reference
The high-resolution crystal structure of porcine pepsinogen., Hartsuck JA, Koelsch G, Remington SJ, Proteins. 1992 May;13(1):1-25. PMID:1594574 Page seeded by OCA on Sun May 4 22:11:10 2008
