1rp0: Difference between revisions
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'''Crystal Structure of Thi1 protein from Arabidopsis thaliana''' | '''Crystal Structure of Thi1 protein from Arabidopsis thaliana''' | ||
==Overview== | |||
Thiamin pyrophosphate is an essential coenzyme in all organisms that depend on fermentation, respiration or photosynthesis to produce ATP. It is synthesized through two independent biosynthetic routes: one for the synthesis of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate (pyrimidine moiety) and another for the synthesis of 4-methyl-5-(beta-hydroxyethyl) thiazole phosphate (thiazole moiety). Herein, we will describe the three-dimensional structure of THI1 protein from Arabidopsis thaliana determined by single wavelength anomalous diffraction to 1.6A resolution. The protein was produced using heterologous expression in bacteria, unexpectedly bound to 2-carboxylate-4-methyl-5-beta-(ethyl adenosine 5-diphosphate) thiazole, a potential intermediate of the thiazole biosynthesis in Eukaryotes. THI1 has a topology similar to dinucleotide binding domains and although details concerning its function are unknown, this work provides new clues about the thiazole biosynthesis in Eukaryotes. | |||
==About this Structure== | ==About this Structure== | ||
1RP0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RP0 OCA]. | 1RP0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RP0 OCA]. | ||
==Reference== | |||
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana., Godoi PH, Galhardo RS, Luche DD, Van Sluys MA, Menck CF, Oliva G, J Biol Chem. 2006 Oct 13;281(41):30957-66. Epub 2006 Aug 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16912043 16912043] | |||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Oliva, G.]] | [[Category: Oliva, G.]] | ||
[[Category: Sluys, M A.Van.]] | [[Category: Sluys, M A.Van.]] | ||
[[Category: Biosynthetic protein]] | |||
[[Category: Protein ligand complex]] | [[Category: Protein ligand complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:16:23 2008'' | ||
