Rop protein: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 6: | Line 6: | ||
== Mutants == | == Mutants == | ||
Numerous mutations in the loop region of Rop have been produced (deletion of 5 a/a of loop, site-directed mutants in loop, replacement and insertion of glycine residues in loop, restored of heptad pattern in loop region). | Numerous mutations in the loop region of Rop have been produced (deletion of 5 a/a of loop ([[1b6q]]), site-directed mutants in loop, replacement and insertion of glycine residues in loop, restored of heptad pattern in loop region). | ||
A31P appears to be trapped in a molten globule state, the reason being that Pro31, unlike Ala31, is more confor-mationally constrained (the dihedral angles of Ala31 in the wild type molecule are prohibited to Pro) and leads to a folding pathway for a thermodynamically less stable confor-mation. | A31P appears to be trapped in a molten globule state, the reason being that Pro31, unlike Ala31, is more confor-mationally constrained (the dihedral angles of Ala31 in the wild type molecule are prohibited to Pro) and leads to a folding pathway for a thermodynamically less stable confor-mation. | ||
