2inv: Difference between revisions
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'''Crystal structure of inulin fructotransferase in the presence of di-fructose''' | '''Crystal structure of inulin fructotransferase in the presence of di-fructose''' | ||
==Overview== | |||
Inulin fructotransferase (IFTase), a member of glycoside hydrolase family 91, catalyzes depolymerization of beta-2,1-fructans inulin by successively removing the terminal difructosaccharide units as cyclic anhydrides via intramolecular fructosyl transfer. The crystal structures of IFTase and its substrate-bound complex reveal that IFTase is a trimeric enzyme, and each monomer folds into a right-handed parallel beta-helix. Despite variation in the number and conformation of its beta-strands, the IFTase beta-helix has a structure that is largely reminiscent of other beta-helix structures but is unprecedented in that trimerization is a prerequisite for catalytic activity, and the active site is located at the monomer-monomer interface. Results from crystallographic studies and site-directed mutagenesis provide a structural basis for the exolytic-type activity of IFTase and a functional resemblance to inverting-type glycosyltransferases. | |||
==About this Structure== | ==About this Structure== | ||
2INV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INV OCA]. | 2INV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2INV OCA]. | ||
==Reference== | |||
Structural and functional insights into intramolecular fructosyl transfer by inulin fructotransferase., Jung WS, Hong CK, Lee S, Kim CS, Kim SJ, Kim SI, Rhee S, J Biol Chem. 2007 Mar 16;282(11):8414-23. Epub 2006 Dec 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17192265 17192265] | |||
[[Category: Bacteria]] | [[Category: Bacteria]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Jung, W S.]] | [[Category: Jung, W S.]] | ||
[[Category: Rhee, S.]] | [[Category: Rhee, S.]] | ||
[[Category: Lyase]] | |||
[[Category: Protein-carbohydrate complex]] | [[Category: Protein-carbohydrate complex]] | ||
[[Category: Right-handed parallel beta-helix]] | [[Category: Right-handed parallel beta-helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:15:44 2008'' | ||
