2jfw: Difference between revisions

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==Overview==
{{ABSTRACT_17568739}}
Glutamate racemase is an enzyme essential to the bacterial cell wall biosynthesis pathway, and has therefore been considered as a target for antibacterial drug discovery. We characterized the glutamate racemases of several pathogenic bacteria using structural and biochemical approaches. Here we describe three distinct mechanisms of regulation for the family of glutamate racemases: allosteric activation by metabolic precursors, kinetic regulation through substrate inhibition, and D-glutamate recycling using a d-amino acid transaminase. In a search for selective inhibitors, we identified a series of uncompetitive inhibitors specifically targeting Helicobacter pylori glutamate racemase that bind to a cryptic allosteric site, and used these inhibitors to probe the mechanistic and dynamic features of the enzyme. These structural, kinetic and mutational studies provide insight into the physiological regulation of these essential enzymes and provide a basis for designing narrow-spectrum antimicrobial agents.


==About this Structure==
==About this Structure==
2JFW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JFW OCA].  
2JFW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JFW OCA].  
==Reference==
Exploitation of structural and regulatory diversity in glutamate racemases., Lundqvist T, Fisher SL, Kern G, Folmer RH, Xue Y, Newton DT, Keating TA, Alm RA, de Jonge BL, Nature. 2007 Jun 14;447(7146):817-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17568739 17568739]
[[Category: Enterococcus faecium]]
[[Category: Enterococcus faecium]]
[[Category: Glutamate racemase]]
[[Category: Glutamate racemase]]
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[[Category: Peptidoglycan biosynthesis]]
[[Category: Peptidoglycan biosynthesis]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 15:06:25 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 18:39:05 2008''

Revision as of 15:39, 25 June 2008

File:2jfw.png

Template:STRUCTURE 2jfw

CRYSTAL STRUCTURE OF ENTEROCOCCUS FAECIUM GLUTAMATE RACEMASE IN COMPLEX WITH TARTRATE


Template:ABSTRACT 17568739

About this Structure

2JFW is a Single protein structure of sequence from Enterococcus faecium. Full crystallographic information is available from OCA.

Page seeded by OCA on Wed Jun 25 18:39:05 2008

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