1cll: Difference between revisions

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[[Image:1cll.gif|left|200px]]
[[Image:1cll.png|left|200px]]


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==Overview==
{{ABSTRACT_1474585}}
We have determined and refined the crystal structure of a recombinant calmodulin at 1.7 A resolution. The structure was determined by molecular replacement, using the 2.2 A published native bovine brain structure as the starting model. The final crystallographic R-factor, using 14,469 reflections in the 10.0 to 1.7 A range with structure factors exceeding 0.5 sigma, is 0.216. Bond lengths and bond angle distances have root-mean-square deviations from ideal values of 0.009 A and 0.032 A, respectively. The final model consists of 1279 non-hydrogen atoms, including four calcium ions, 1130 protein atoms, including three Asp118 side-chain atoms in double conformation, 139 water molecules and one ethanol molecule. The electron densities for residues 1 to 4 and 148 of calmodulin are poorly defined, and not included in our model, except for main-chain atoms of residue 4. The calmodulin structure from our crystals is very similar to the earlier 2.2 A structure described by Babu and coworkers with a root-mean-square deviation of 0.36 A. Calmodulin remains a dumb-bell-shaped molecule, with similar lobes and connected by a central alpha-helix. Each lobe contains three alpha-helices and two Ca2+ binding EF hand loops, with a short antiparallel beta-sheet between adjacent EF hand loops and one non-EF hand loop. There are some differences in the structure of the central helix. The crystal packing is extensively studied, and facile crystal growth along the z-axis of the triclinic crystals is explained. Herein, we describe hydrogen bonding in the various secondary structure elements and hydration of calmodulin.


==About this Structure==
==About this Structure==
1CLL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1CLL with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb44_1.html Calmodulin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLL OCA].  
1CLL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The following page contains interesting information on the relation of 1CLL with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb44_1.html Calmodulin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CLL OCA].  
==Reference==
Calmodulin structure refined at 1.7 A resolution., Chattopadhyaya R, Meador WE, Means AR, Quiocho FA, J Mol Biol. 1992 Dec 20;228(4):1177-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1474585 1474585]
[[Category: Calmodulin]]
[[Category: Calmodulin]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Quiocho, F A.]]
[[Category: Quiocho, F A.]]
[[Category: Calcium-binding protein]]
[[Category: Calcium-binding protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:51:56 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:08:29 2008''

Revision as of 14:08, 26 June 2008

File:1cll.png

Template:STRUCTURE 1cll

CALMODULIN STRUCTURE REFINED AT 1.7 ANGSTROMS RESOLUTION


Template:ABSTRACT 1474585

About this Structure

1CLL is a Single protein structure of sequence from Homo sapiens. The following page contains interesting information on the relation of 1CLL with [Calmodulin]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:08:29 2008

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