1i6h: Difference between revisions

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[[Image:1i6h.jpg|left|200px]]
[[Image:1i6h.png|left|200px]]


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==Overview==
{{ABSTRACT_11313499}}
The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 A resolution. Duplex DNA is seen entering the main cleft of the enzyme and unwinding before the active site. Nine base pairs of DNA-RNA hybrid extend from the active center at nearly right angles to the entering DNA, with the 3' end of the RNA in the nucleotide addition site. The 3' end is positioned above a pore, through which nucleotides may enter and through which RNA may be extruded during back-tracking. The 5'-most residue of the RNA is close to the point of entry to an exit groove. Changes in protein structure between the transcribing complex and free enzyme include closure of a clamp over the DNA and RNA and ordering of a series of "switches" at the base of the clamp to create a binding site complementary to the DNA-RNA hybrid. Protein-nucleic acid contacts help explain DNA and RNA strand separation, the specificity of RNA synthesis, "abortive cycling" during transcription initiation, and RNA and DNA translocation during transcription elongation.


==About this Structure==
==About this Structure==
1I6H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 1I6H with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb40_1.html RNA Polymerase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I6H OCA].  
1I6H is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 1I6H with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb40_1.html RNA Polymerase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I6H OCA].  
==Reference==
Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution., Gnatt AL, Cramer P, Fu J, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1876-82. Epub 2001 Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11313499 11313499]
[[Category: DNA-directed RNA polymerase]]
[[Category: DNA-directed RNA polymerase]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Multiprotein complex]]
[[Category: Multiprotein complex]]
[[Category: Transcription]]
[[Category: Transcription]]
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