1jku: Difference between revisions

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[[Image:1jku.gif|left|200px]]
[[Image:1jku.png|left|200px]]


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==Overview==
{{ABSTRACT_11587647}}
BACKGROUND: Catalases are important antioxidant metalloenzymes that catalyze disproportionation of hydrogen peroxide, forming dioxygen and water. Two families of catalases are known, one having a heme cofactor, and the other, a structurally distinct family containing nonheme manganese. We have solved the structure of the mesophilic manganese catalase from Lactobacillus plantarum and its azide-inhibited complex. RESULTS: The crystal structure of the native enzyme has been solved at 1.8 A resolution by molecular replacement, and the azide complex of the native protein has been solved at 1.4 A resolution. The hexameric structure of the holoenzyme is stabilized by extensive intersubunit contacts, including a beta zipper and a structural calcium ion crosslinking neighboring subunits. Each subunit contains a dimanganese active site, accessed by a single substrate channel lined by charged residues. The manganese ions are linked by a mu1,3-bridging glutamate carboxylate and two mu-bridging solvent oxygens that electronically couple the metal centers. The active site region includes two residues (Arg147 and Glu178) that appear to be unique to the Lactobacillus plantarum catalase. CONCLUSIONS: A comparison of L. plantarum and T. thermophilus catalase structures reveals the existence of two distinct structural classes, differing in monomer design and the organization of their active sites, within the manganese catalase family. These differences have important implications for catalysis and may reflect distinct biological functions for the two enzymes, with the L. plantarum enzyme serving as a catalase, while the T. thermophilus enzyme may function as a catalase/peroxidase.


==About this Structure==
==About this Structure==
1JKU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum]. The following page contains interesting information on the relation of 1JKU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb57_1.html Catalase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKU OCA].  
1JKU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum]. The following page contains interesting information on the relation of 1JKU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb57_1.html Catalase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKU OCA].  
==Reference==
Crystal structure of manganese catalase from Lactobacillus plantarum., Barynin VV, Whittaker MM, Antonyuk SV, Lamzin VS, Harrison PM, Artymiuk PJ, Whittaker JW, Structure. 2001 Aug;9(8):725-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11587647 11587647]
[[Category: Catalase]]
[[Category: Catalase]]
[[Category: Lactobacillus plantarum]]
[[Category: Lactobacillus plantarum]]
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[[Category: Hexamer]]
[[Category: Hexamer]]
[[Category: Metalloenzyme]]
[[Category: Metalloenzyme]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:20:38 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:36:04 2008''

Revision as of 14:36, 26 June 2008

File:1jku.png

Template:STRUCTURE 1jku

Crystal Structure of Manganese Catalase from Lactobacillus plantarum


Template:ABSTRACT 11587647

About this Structure

1JKU is a Single protein structure of sequence from Lactobacillus plantarum. The following page contains interesting information on the relation of 1JKU with [Catalase]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:36:04 2008

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