1l35: Difference between revisions

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[[Image:1l35.jpg|left|200px]]
[[Image:1l35.png|left|200px]]


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==Overview==
{{ABSTRACT_2334683}}
A disulfide bond introduced between amino acid positions 9 and 164 in phage T4 lysozyme has been shown to significantly increase the stability of the enzyme toward thermal denaturation [Matsumura, M., Becktel, W.J., Levitt, M., &amp; Matthews, B. W. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 6562-6566]. To elucidate the structural features of the engineered disulfide, the crystal structure of the disulfide mutant has been determined at 1.8-A resolution. Residue 9 lies in the N-terminal alpha-helix, while residue 164 is located at the extreme C terminus of T4 lysozyme, which is the most mobile part of the molecule. The refined structure shows that the formation of the disulfide bond is accompanied by relatively large (approximately 2.5 A) localized shifts in C-terminal main-chain atoms. Comparison of the geometry of the engineered disulfide with those of naturally observed disulfides in proteins shows that the engineered bridge adopts a left-handed spiral conformation with a typical set of dihedral angles and C alpha-C alpha distance. The geometry of the engineered disulfide suggests that it is slightly more strained than the disulfide of oxidized dithiothreitol but that the strain is within the range observed in naturally occurring disulfides. The wild-type and cross-linked lysozymes have very similar overall crystallographic temperature factors, indicating that the introduction of the disulfide bond does not impose rigidity on the folded protein structure. In particular, residues 162-164 retain high mobility in the mutant structure, consistent with the idea that stabilization of the protein is due to the effect of the disulfide cross-link on the unfolded rather than the folded state.(ABSTRACT TRUNCATED AT 250 WORDS)


==About this Structure==
==About this Structure==
1L35 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. The following page contains interesting information on the relation of 1L35 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb9_1.html Lysozyme]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L35 OCA].  
1L35 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. The following page contains interesting information on the relation of 1L35 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb9_1.html Lysozyme]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L35 OCA].  
==Reference==
Structure of a thermostable disulfide-bridge mutant of phage T4 lysozyme shows that an engineered cross-link in a flexible region does not increase the rigidity of the folded protein., Pjura PE, Matsumura M, Wozniak JA, Matthews BW, Biochemistry. 1990 Mar 13;29(10):2592-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2334683 2334683]
[[Category: Enterobacteria phage t4]]
[[Category: Enterobacteria phage t4]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
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[[Category: Pjura, P E.]]
[[Category: Pjura, P E.]]
[[Category: Wozniak, J A.]]
[[Category: Wozniak, J A.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:29:15 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:40:12 2008''

Revision as of 14:40, 26 June 2008

File:1l35.png

Template:STRUCTURE 1l35

STRUCTURE OF A THERMOSTABLE DISULFIDE-BRIDGE MUTANT OF PHAGE T4 LYSOZYME SHOWS THAT AN ENGINEERED CROSSLINK IN A FLEXIBLE REGION DOES NOT INCREASE THE RIGIDITY OF THE FOLDED PROTEIN


Template:ABSTRACT 2334683

About this Structure

1L35 is a Single protein structure of sequence from Enterobacteria phage t4. The following page contains interesting information on the relation of 1L35 with [Lysozyme]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:40:12 2008

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