1nsf: Difference between revisions

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[[Image:1nsf.gif|left|200px]]
[[Image:1nsf.png|left|200px]]


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==Overview==
{{ABSTRACT_9731775}}
N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase which primes and/or dissociates SNARE complexes involved in intracellular fusion events. Each NSF protomer contains three domains: an N-terminal domain required for SNARE binding and two ATPase domains, termed D1 and D2, with D2 being required for oligomerization. We have determined the 1.9 A crystal structure of the D2 domain of NSF complexed with ATP using multi-wavelength anomalous dispersion phasing. D2 consists of a nucleotide binding subdomain with a Rossmann fold and a C-terminal subdomain, which is structurally unique among nucleotide binding proteins. There are interactions between the ATP moiety and both the neighboring D2 protomer and the C-terminal subdomain that may be important for ATP-dependent oligomerization. Of particular importance are three well-ordered and conserved lysine residues that form ionic interactions with the beta- and gamma-phosphates, one of which likely contributes to the low hydrolytic activity of D2.


==About this Structure==
==About this Structure==
1NSF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. The following page contains interesting information on the relation of 1NSF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSF OCA].  
1NSF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. The following page contains interesting information on the relation of 1NSF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSF OCA].  
==Reference==
Structure of the ATP-dependent oligomerization domain of N-ethylmaleimide sensitive factor complexed with ATP., Yu RC, Hanson PI, Jahn R, Brunger AT, Nat Struct Biol. 1998 Sep;5(9):803-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9731775 9731775]
[[Category: AAA+ Proteases]]
[[Category: AAA+ Proteases]]
[[Category: Cricetulus griseus]]
[[Category: Cricetulus griseus]]
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[[Category: Golgi stack]]
[[Category: Golgi stack]]
[[Category: Protein transport]]
[[Category: Protein transport]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:55:19 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:45:49 2008''

Revision as of 14:45, 26 June 2008

File:1nsf.png

Template:STRUCTURE 1nsf

D2 HEXAMERIZATION DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)


Template:ABSTRACT 9731775

About this Structure

1NSF is a Single protein structure of sequence from Cricetulus griseus. The following page contains interesting information on the relation of 1NSF with [AAA+ Proteases]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:45:49 2008

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