2aai: Difference between revisions

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[[Image:2aai.jpg|left|200px]]
[[Image:2aai.png|left|200px]]


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==Overview==
{{ABSTRACT_1881880}}
The plant cytotoxin ricin consists of two disulfide-linked chains, each of about 30,000 daltons. An initial model based on a 2.8 A MIR electron density map has been refined against 2.5 A data using rounds of hand rebuilding coupled with either a restrained least squares algorithm or molecular dynamics (XPLOR). The last model (9) has an R factor of 21.6% and RMS deviations from standard bond lengths and angles of 0.021 A and 4.67 degrees, respectively. Refinement required several peptide segments in the original model to be adjusted translationally along the electron density. A wide range of lesser changes were also made. The RMS deviation of backbone atoms between the original and model 9 was 1.89 A. Molecular dynamics proved to be a very powerful refinement tool. However, tests showed that it could not replace human intervention in making adjustments such as local translations of the peptide chain. The R factor is not a completely satisfactory indicator of refinement progress; difference Fouriers, when observed carefully, may be a better monitor.


==About this Structure==
==About this Structure==
2AAI is a [[Single protein]] structure. The following page contains interesting information on the relation of 2AAI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb69_1.html Cholera Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAI OCA].  
2AAI is a [[Single protein]] structure. The following page contains interesting information on the relation of 2AAI with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb69_1.html Cholera Toxin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AAI OCA].  
==Reference==
Crystallographic refinement of ricin to 2.5 A., Rutenber E, Katzin BJ, Ernst S, Collins EJ, Mlsna D, Ready MP, Robertus JD, Proteins. 1991;10(3):240-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1881880 1881880]
[[Category: Cholera Toxin]]
[[Category: Cholera Toxin]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Villafranca, J E.]]
[[Category: Villafranca, J E.]]
[[Category: Glycosidase]]
[[Category: Glycosidase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 18:49:00 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:11:40 2008''

Revision as of 15:11, 26 June 2008

File:2aai.png

Template:STRUCTURE 2aai

CRYSTALLOGRAPHIC REFINEMENT OF RICIN TO 2.5 ANGSTROMS


Template:ABSTRACT 1881880

About this Structure

2AAI is a Single protein structure. The following page contains interesting information on the relation of 2AAI with [Cholera Toxin]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:11:40 2008

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