3cyt: Difference between revisions

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[[Image:3cyt.gif|left|200px]]
[[Image:3cyt.png|left|200px]]


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==Overview==
{{ABSTRACT_6256733}}
Tuna ferrocytochrome c and ferricytochrome c have been refined independently at high resolution (1.5 A and 1.8 A) to crystallographic residual errors of 17.3% and 20.8%, respectively. Small but significant conformational differences are seen surrounding a buried water molecule that is hydrogen bonded to Asn-52, Tyr-67, and Thr-78. In the oxidized state, this water molecule is 1.0 A closer to the heme and the heme has moved 0.15 A out of its heme crevice; both changes lead to a more polar microenvironment for the heme. Chemical modification studies, patterns of evolutionary conservatism, structural differences in bacterial cytochromes, and x-ray studies all agree that the "active site" for cytochrome c is bounded by lysines 8, 13,27, 72, 79, 86, and 87 (thus containing the evolutionary conservative 72-87 loop) and has the buried water molecule just below its surface and the opening of the heme crevice slightly to one side.


==About this Structure==
==About this Structure==
3CYT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thunnus_alalunga Thunnus alalunga]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cyt 1cyt]. The following pages contain interesting information on the relation of 3CYT with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb89_1.html Aconitase and Iron Regulatory Protein 1]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CYT OCA].  
3CYT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thunnus_alalunga Thunnus alalunga]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cyt 1cyt]. The following pages contain interesting information on the relation of 3CYT with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb89_1.html Aconitase and Iron Regulatory Protein 1]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CYT OCA].  
==Reference==
Redox conformation changes in refined tuna cytochrome c., Takano T, Dickerson RE, Proc Natl Acad Sci U S A. 1980 Nov;77(11):6371-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/6256733 6256733]
[[Category: Aconitase and Iron Regulatory Protein 1]]
[[Category: Aconitase and Iron Regulatory Protein 1]]
[[Category: Cytochrome c]]
[[Category: Cytochrome c]]
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[[Category: Thunnus alalunga]]
[[Category: Thunnus alalunga]]
[[Category: Takano, T.]]
[[Category: Takano, T.]]
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