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[[Image:3est.gif|left|200px]]
[[Image:3est.png|left|200px]]


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==Overview==
{{ABSTRACT_3271103}}
The structure of native porcine pancreatic elastase in 70% methanol has been refined using film data to 1.65 A resolution, R = 0.169. A total of 134 molecules of water (but no methanol) has been refined. This structure, because of its native state and modestly high resolution, serves as the basis for comparison with other elastase structures complexed with natural or synthetic ligands. Internal structured water occupies distinct regions. Two regions (IW1 and IW7) suggest a mechanism for equalizing 'hydrostatic pressure' related to ligand binding and release. A third region (IW4) forms part of a hydrogen-bonding network linking the catalytic Ser 195 O gamma with a remote (13.4 A) surface of the enzyme. A comparison with the structures of all known serine proteases reveals that a linkage of Ser O gamma to remote surface is conserved in all cases, suggesting that the accepted catalytic mechanism of serine proteases needs to be re-evaluated. One possible mechanism for base catalysis of Ser O gamma H proton extraction is presented.


==About this Structure==
==About this Structure==
3EST is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. The following page contains interesting information on the relation of 3EST with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb46_1.html Trypsin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EST OCA].  
3EST is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. The following page contains interesting information on the relation of 3EST with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb46_1.html Trypsin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EST OCA].  
==Reference==
Structure of native porcine pancreatic elastase at 1.65 A resolutions., Meyer E, Cole G, Radhakrishnan R, Epp O, Acta Crystallogr B. 1988 Feb 1;44 ( Pt 1):26-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3271103 3271103]
[[Category: Pancreatic elastase]]
[[Category: Pancreatic elastase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Meyer, E F.]]
[[Category: Meyer, E F.]]
[[Category: Radhakrishnan, R.]]
[[Category: Radhakrishnan, R.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:01:16 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:26:21 2008''

Revision as of 15:26, 26 June 2008

File:3est.png

Template:STRUCTURE 3est

STRUCTURE OF NATIVE PORCINE PANCREATIC ELASTASE AT 1.65 ANGSTROMS RESOLUTION


Template:ABSTRACT 3271103

About this Structure

3EST is a Single protein structure of sequence from Sus scrofa. The following page contains interesting information on the relation of 3EST with [Trypsin]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:26:21 2008

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