9rub: Difference between revisions

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[[Image:9rub.gif|left|200px]]
[[Image:9rub.png|left|200px]]


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==Overview==
{{ABSTRACT_1905726}}
The three-dimensional structure of the complex of ribulose-1,5-bisphosphate carboxylase from Rhodospirillum rubrum, CO2, Mg2+, and ribulose bisphosphate has been determined with x-ray crystallographic methods to 2.6-A resolution. Ribulose-1,5-bisphosphate binds across the active site with the two phosphate groups in the two phosphate binding sites of the beta/alpha barrel. The oxygen atoms of the carbamate and the side chain of Asp-193 provide the protein ligands to the bound Mg2+ ion. The C2 and the C3 or C4 oxygen atoms of the substrate are also within the first coordination sphere of the metal ion. At the present resolution of the electron density maps, two slightly different conformations of the substrate, with the C3 hydroxyl group "cis" or "trans" to the C2 oxygen, can be built into the observed electron density. The two different conformations suggest two different mechanisms of proton abstraction in the first step of catalysis, the enolization of the ribulose 1,5-bisphosphate. Two loop regions, which are disordered in the crystals of the nonactivated enzyme, could be built into their respective electron density. A comparison with the structure of the quaternary complex of the spinach enzyme shows that despite the different conformations of loop 6, the positions of the Mg2+ ion, and most atoms of the substrate are very similar when superimposed on each other. There are, however, some significant differences at the active site, especially in the metal coordination sphere.


==About this Structure==
==About this Structure==
9RUB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. The following page contains interesting information on the relation of 9RUB with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb11_1.html Rubisco]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9RUB OCA].  
9RUB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. The following page contains interesting information on the relation of 9RUB with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb11_1.html Rubisco]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9RUB OCA].  
==Reference==
Crystal structure of activated ribulose-1,5-bisphosphate carboxylase complexed with its substrate, ribulose-1,5-bisphosphate., Lundqvist T, Schneider G, J Biol Chem. 1991 Jul 5;266(19):12604-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1905726 1905726]
[[Category: Rhodospirillum rubrum]]
[[Category: Rhodospirillum rubrum]]
[[Category: Ribulose-bisphosphate carboxylase]]
[[Category: Ribulose-bisphosphate carboxylase]]
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[[Category: Lundqvist, T.]]
[[Category: Lundqvist, T.]]
[[Category: Schneider, G.]]
[[Category: Schneider, G.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:55:12 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:32:06 2008''

Revision as of 15:32, 26 June 2008

File:9rub.png

Template:STRUCTURE 9rub

CRYSTAL STRUCTURE OF ACTIVATED RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE COMPLEXED WITH ITS SUBSTRATE, RIBULOSE-1,5-BISPHOSPHATE


Template:ABSTRACT 1905726

About this Structure

9RUB is a Single protein structure of sequence from Rhodospirillum rubrum. The following page contains interesting information on the relation of 9RUB with [Rubisco]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:32:06 2008

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