1a7j: Difference between revisions

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[[Image:1a7j.gif|left|200px]]
{{Seed}}
[[Image:1a7j.png|left|200px]]


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{{STRUCTURE_1a7j|  PDB=1a7j  |  SCENE=  }}  
{{STRUCTURE_1a7j|  PDB=1a7j  |  SCENE=  }}  


'''PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES'''
===PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES===




==Overview==
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The essential photosynthetic enzyme phosphoribulokinase (PRK) is responsible for the conversion of ribulose 5-phosphate (Ru5P) to ribulose 1,5-bisphosphate, the substrate for the CO2 fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco). We have determined the structure of the octameric bacterial form of PRK to a resolution of 2.5 A. The protein is folded into a seven-member mixed beta-sheet surrounded by alpha-helices, giving the overall appearance of the nucleotide monophosphate family of kinases. Homology with the nucleotide monophosphate kinases suggests a number of amino acid residues that are likely to be important in catalysis and suggests the roles of some amino acid residues that have been mutated prior to the determination of the structure. Further, sequence identity across eukaryotic and prokaryotic species and a calculation of the buried surface area suggests the identity within the octamer of a dimer conserved throughout evolution. The width of the groove leading to the active site is consistent with an oriented molecule of thioredoxin controlling the oxidation state of two cysteines that regulate activity in the eukaryotic enzymes. Although neither Asp 42 nor Asp 169 can be definitively assigned as the catalytic base, the crystal structure suggests the location of a ribulose 5-phosphate binding site and suggests a role for several of the conserved basic residues.
The line below this paragraph, {{ABSTRACT_PUBMED_9548738}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9548738 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9548738}}


==About this Structure==
==About this Structure==
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[[Category: Kinase]]
[[Category: Kinase]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 13:18, 30 June 2008

File:1a7j.png

Template:STRUCTURE 1a7j

PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES

Template:ABSTRACT PUBMED 9548738

About this Structure

1A7J is a Single protein structure of sequence from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

Reference

The crystal structure of phosphoribulokinase from Rhodobacter sphaeroides reveals a fold similar to that of adenylate kinase., Harrison DH, Runquist JA, Holub A, Miziorko HM, Biochemistry. 1998 Apr 14;37(15):5074-85. PMID:9548738

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