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| [[Image:1aj3.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1aj3| PDB=1aj3 | SCENE= }} | | {{STRUCTURE_1aj3| PDB=1aj3 | SCENE= }} |
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| '''SOLUTION STRUCTURE OF THE SPECTRIN REPEAT, NMR, 20 STRUCTURES'''
| | ===SOLUTION STRUCTURE OF THE SPECTRIN REPEAT, NMR, 20 STRUCTURES=== |
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| ==Overview==
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| Cytoskeletal proteins belonging to the spectrin family have an elongated structure composed of repetitive units. The three-dimensional solution structure of the 16th repeat from chicken brain alpha-spectrin (R16) has been determined by NMR spectroscopy and distance geometry-simulated annealing calculations. We used a total of 1035 distance restraints, which included 719 NOE-based values obtained by applying the ambiguous restraints for iterative assignment (ARIA) method. In addition, we performed a direct refinement against 1H-chemical shifts. The final ensemble of 20 structures shows an average RMSD of 1.52 A from the mean for the backbone atoms, excluding loops and N and C termini. R16 is made up of three antiparallel alpha-helices separated by two loops, and folds into a left-handed coiled-coil.The basic unit of spectrin is an antiparallel heterodimer composed of two homologous chains, beta and alpha. These assemble a tetramer via a mechanism that relies on the completion of a single repeat by association of the partial repeats located at the C terminus of the beta-chain (two helices) and at the N terminus of the alpha-chain (one helix). This tetramer is the assemblage able to cross-link actin filaments. Model building by homology of the "tetramerization" repeat from human erythrocyte spectrin illuminates the possible role of point mutations which cause hemolytic anemias.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9356261}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9356261 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9356261}} |
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| ==About this Structure== | | ==About this Structure== |
| 1AJ3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJ3 OCA]. | | 1AJ3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJ3 OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Sh3 domain]] | | [[Category: Sh3 domain]] |
| [[Category: Spectrin]] | | [[Category: Spectrin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:20:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:57:23 2008'' |
