1ayp: Difference between revisions

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[[Image:1ayp.gif|left|200px]]
{{Seed}}
[[Image:1ayp.png|left|200px]]


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{{STRUCTURE_1ayp|  PDB=1ayp  |  SCENE=  }}  
{{STRUCTURE_1ayp|  PDB=1ayp  |  SCENE=  }}  


'''A PROBE MOLECULE COMPOSED OF SEVENTEEN PERCENT OF TOTAL DIFFRACTING MATTER GIVES CORRECT SOLUTIONS IN MOLECULAR REPLACEMENT'''
===A PROBE MOLECULE COMPOSED OF SEVENTEEN PERCENT OF TOTAL DIFFRACTING MATTER GIVES CORRECT SOLUTIONS IN MOLECULAR REPLACEMENT===




==Overview==
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It is often found in the crystallization of enzyme-inhibitor complexes that an inhibitor causes crystal packing which is different to that of native protein. This is the case for crystals of human non-pancreatic secreted phospholipase A(2) (124 residues) containing six molecules in the asymmetric unit when the protein is complexed with a potential acylamino analogue of a phospholid. The hexameric structure was determined by molecular replacement using the structure of monomeric native protein as a probe. As an extension to the experiment, it was tested whether a backbone polypeptide composed of 17% of a known monomeric structure could find its correct position on a target molecule in molecular replacement. A probe model composed of the backbone atoms of the N-terminal 77 residues of lysine-, arginine-, ornithine-binding protein (LAO, a total of 238 residues) liganded with lysine correctly finds its position on LAO liganded with histidine which crystallizes as a monomer in the asymmetric unit. The results indicate that as little as 17% of total diffracting matter can be used in molecular replacement to solve crystal structures or to obtain phase information which can be combined with phases obtained by the isomorphous-replacement method.
The line below this paragraph, {{ABSTRACT_PUBMED_15299314}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_15299314}}


==About this Structure==
==About this Structure==
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[[Category: Oh, B H.]]
[[Category: Oh, B H.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:51:24 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:52:29 2008''

Revision as of 14:52, 30 June 2008

File:1ayp.png

Template:STRUCTURE 1ayp

A PROBE MOLECULE COMPOSED OF SEVENTEEN PERCENT OF TOTAL DIFFRACTING MATTER GIVES CORRECT SOLUTIONS IN MOLECULAR REPLACEMENT

Template:ABSTRACT PUBMED 15299314

About this Structure

1AYP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

A probe molecule composed of seventeen percent of total diffracting matter gives correct solutions in molecular replacement., Oh BH, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):140-4. PMID:15299314

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