1b9h: Difference between revisions

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[[Image:1b9h.gif|left|200px]]
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[[Image:1b9h.png|left|200px]]


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{{STRUCTURE_1b9h|  PDB=1b9h  |  SCENE=  }}  
{{STRUCTURE_1b9h|  PDB=1b9h  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF 3-AMINO-5-HYDROXYBENZOIC ACID (AHBA) SYNTHASE'''
===CRYSTAL STRUCTURE OF 3-AMINO-5-HYDROXYBENZOIC ACID (AHBA) SYNTHASE===




==Overview==
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The biosynthesis of ansamycin antibiotics, including rifamycin B, involves the synthesis of an aromatic precursor, 3-amino-5-hydroxybenzoic acid (AHBA), which serves as starter for the assembly of the antibiotics' polyketide backbone. The terminal enzyme of AHBA formation, AHBA synthase, is a dimeric, pyridoxal 5'-phosphate (PLP) dependent enzyme with pronounced sequence homology to a number of PLP enzymes involved in the biosynthesis of antibiotic sugar moieties. The structure of AHBA synthase from Amycolatopsis mediterranei has been determined to 2.0 A resolution, with bound cofactor, PLP, and in a complex with PLP and an inhibitor (gabaculine). The overall fold of AHBA synthase is similar to that of the aspartate aminotransferase family of PLP-dependent enzymes, with a large domain containing a seven-stranded beta-sheet surrounded by alpha-helices and a smaller domain consisting of a four-stranded antiparallel beta-sheet and four alpha-helices. The uninhibited form of the enzyme shows the cofactor covalently linked to Lys188 in an internal aldimine linkage. On binding the inhibitor, gabaculine, the internal aldimine linkage is broken, and a covalent bond is observed between the cofactor and inhibitor. The active site is composed of residues from two subunits of AHBA synthase, indicating that AHBA synthase is active as a dimer.
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{{ABSTRACT_PUBMED_10433690}}


==About this Structure==
==About this Structure==
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[[Category: Scapin, G.]]
[[Category: Scapin, G.]]
[[Category: Yu, T W.]]
[[Category: Yu, T W.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:37:58 2008''

Revision as of 15:38, 30 June 2008

File:1b9h.png

Template:STRUCTURE 1b9h

CRYSTAL STRUCTURE OF 3-AMINO-5-HYDROXYBENZOIC ACID (AHBA) SYNTHASE

Template:ABSTRACT PUBMED 10433690

About this Structure

1B9H is a Single protein structure of sequence from Amycolatopsis mediterranei. Full crystallographic information is available from OCA.

Reference

Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase., Eads JC, Beeby M, Scapin G, Yu TW, Floss HG, Biochemistry. 1999 Aug 3;38(31):9840-9. PMID:10433690

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