1bcu: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
Line 1: Line 1:
[[Image:1bcu.jpg|left|200px]]
{{Seed}}
[[Image:1bcu.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1bcu|  PDB=1bcu  |  SCENE=  }}  
{{STRUCTURE_1bcu|  PDB=1bcu  |  SCENE=  }}  


'''ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND PROFLAVIN'''
===ALPHA-THROMBIN COMPLEXED WITH HIRUGEN AND PROFLAVIN===




==Overview==
<!--
Proflavin can be used to study the interactions of inhibitors and substrates with thrombin by monitoring the changes in the visible absorption spectrum that occur on dye displacement. We have used microspectrophotometric methods to investigate the binding of proflavin to crystals of an alpha-thrombin-hirugen complex and have determined the structure by X-ray crystallography. The proflavin molecule binds in the S1 pocket of the enzyme with one of the amino groups hydrogen bonded to the carboxylate of Asp-189 while the protonated ring nitrogen is hydrogen bonded to the carbonyl of Gly-219. This result indicates that the proflavin displacement assay can be used to specifically monitor the binding of inhibitors to the S1 pocket.
The line below this paragraph, {{ABSTRACT_PUBMED_9559654}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9559654 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_9559654}}


==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Serine protease]]
[[Category: Serine protease]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:20:57 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:51:07 2008''
Retrieved from "https://proteopedia.org/1bcu"