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| {{STRUCTURE_1bk2| PDB=1bk2 | SCENE= }} | | {{STRUCTURE_1bk2| PDB=1bk2 | SCENE= }} |
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| '''A-SPECTRIN SH3 DOMAIN D48G MUTANT'''
| | ===A-SPECTRIN SH3 DOMAIN D48G MUTANT=== |
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| ==Overview==
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| We have analyzed the existence of obligatory steps in the folding reaction of the alpha-spectrin SH3 domain by mutating Asp 48 (D48G), which is at position i+3 of an isolated two-residue type II' beta-turn. Calorimetry and X-ray analysis show an entropic stabilizing effect resulting from local changes at the dihedral angles of the beta-turn. Kinetic analysis of D48G shows that this beta-turn is fully formed in the transition state, while there is no evidence of its formation in an isolated fragment. Introduction of several mutations in the D48G protein reveals that the local stabilization has not significantly altered the transition state ensemble. All these results, together with previous analysis of other alpha-spectrin and src SH3 mutants, indicate that: (i) in the folding reaction there could be obligatory steps which are not necessarily part of the folding nucleus; (ii) transition state ensembles in beta-sheet proteins could be quite defined and conformationally restricted ('mechanic folding nucleus'); and (iii) transition state ensembles in some proteins could be evolutionarily conserved.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9699637}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9699637 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9699637}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Cytoskeleton]] | | [[Category: Cytoskeleton]] |
| [[Category: Sh3-domain]] | | [[Category: Sh3-domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:37:05 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:17:28 2008'' |
