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| {{STRUCTURE_1bkh| PDB=1bkh | SCENE= }} | | {{STRUCTURE_1bkh| PDB=1bkh | SCENE= }} |
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| '''MUCONATE LACTONIZING ENZYME FROM PSEUDOMONAS PUTIDA'''
| | ===MUCONATE LACTONIZING ENZYME FROM PSEUDOMONAS PUTIDA=== |
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| ==Overview==
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| Muconate lactonizing enzyme (MLE), a component of the beta-ketoadipate pathway of Pseudomonas putida, is a member of a family of related enzymes (the "enolase superfamily") that catalyze the abstraction of the alpha-proton of a carboxylic acid in the context of different overall reactions. New untwinned crystal forms of MLE were obtained, one of which diffracts to better than 2.0-A resolution. The packing of the octameric enzyme in this crystal form is unusual, because the asymmetric unit contains three subunits. The structure of MLE presented here contains no bound metal ion, but is very similar to a recently determined Mn2+-bound structure. Thus, absence of the metal ion does not perturb the structure of the active site. The structures of enolase, mandelate racemase, and MLE were superimposed. A comparison of metal ligands suggests that enolase may retain some characteristics of the ancestor of this enzyme family. Comparison of other residues involved in catalysis indicates two unusual patterns of conservation: (i) that the position of catalytic atoms remains constant, although the residues that contain them are located at different points in the protein fold; and (ii) that the positions of catalytic residues in the protein scaffold are conserved, whereas their identities and roles in catalysis vary.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9724714}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9724714 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9724714}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Muconate cycloisomerase aromatic hydrocarbons catabolism]] | | [[Category: Muconate cycloisomerase aromatic hydrocarbons catabolism]] |
| [[Category: Muconate lactonizing enzyme]] | | [[Category: Muconate lactonizing enzyme]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:37:59 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:18:26 2008'' |
