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| {{STRUCTURE_1bos| PDB=1bos | SCENE= }} | | {{STRUCTURE_1bos| PDB=1bos | SCENE= }} |
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| '''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR'''
| | ===SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR=== |
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| ==Overview==
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| Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9485303}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9485303 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9485303}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Receptor binding]] | | [[Category: Receptor binding]] |
| [[Category: Toxin]] | | [[Category: Toxin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:46:38 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:28:31 2008'' |
