1bsu: Difference between revisions

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[[Image:1bsu.~~gif~~|left|200px]]

[[Image:1bsu.png|left|200px]]

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~~'''~~STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE~~'''~~

===STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE===

~~==Overview==~~

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~~Specific recognition by EcoRV endonuclease of its cognate~~, ~~sharply bent GATATC site~~ at ~~the center TA step occurs solely via hydrophobic interaction with thymine methyl groups~~. ~~Mechanistic kinetic analyses of base analog-substituted DNAs at this position reveal that direct readout provides 5 kcal mol(-1~~) ~~toward specificity~~, ~~with an additional 6-10 kcal mol(-1) arising from indirect readout~~. ~~Crystal structures of several base analog complexes show that the major~~-~~groove hydrophobic contacts are crucial to forming required divalent metal~~-~~binding sites, and that indirect readout operates in part through the sequence-dependent free-energy cost of unstacking the center base-pair step of the DNA.~~

The line below this paragraph, {{ABSTRACT_PUBMED_10074946}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 10074946 is the PubMed ID number.

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==About this Structure==

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[[Category: Martin, A M.]]

[[Category: Perona, J J.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May 2 11~~:54:55 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:40:16 2008''

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-[[Image:1bsu.gif|left|200px]]
+{{Seed}}
+[[Image:1bsu.png|left|200px]]
 <!--
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 {{STRUCTURE_1bsu|  PDB=1bsu  |  SCENE=  }}
-'''STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE'''
+===STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE===
-==Overview==
+<!--
-Specific recognition by EcoRV endonuclease of its cognate, sharply bent GATATC site at the center TA step occurs solely via hydrophobic interaction with thymine methyl groups. Mechanistic kinetic analyses of base analog-substituted DNAs at this position reveal that direct readout provides 5 kcal mol(-1) toward specificity, with an additional 6-10 kcal mol(-1) arising from indirect readout. Crystal structures of several base analog complexes show that the major-groove hydrophobic contacts are crucial to forming required divalent metal-binding sites, and that indirect readout operates in part through the sequence-dependent free-energy cost of unstacking the center base-pair step of the DNA.
+The line below this paragraph, {{ABSTRACT_PUBMED_10074946}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10074946 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10074946}}
 ==About this Structure==
@@ Line 25: / Line 29: @@
 [[Category: Martin, A M.]]
 [[Category: Perona, J J.]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:54:55 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:40:16 2008''