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[[Image:1bx5.gif|left|200px]]
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{{STRUCTURE_1bx5|  PDB=1bx5  |  SCENE=  }}  
{{STRUCTURE_1bx5|  PDB=1bx5  |  SCENE=  }}  


'''NMR SOLUTION STRUCTURE OF [D(GCGAAT-3'-3'-ALPHAT-5'-5'-CGC)2]'''
===NMR SOLUTION STRUCTURE OF [D(GCGAAT-3'-3'-ALPHAT-5'-5'-CGC)2]===




==Overview==
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We present the high-resolution solution structures of a self-complementary DNA decamer duplex featuring a single alpha-anomeric nucleotide per strand encompassed by a set of 3'-3' and 5'-5' phosphodiester linkages, d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2, alphaT, and its unmodified control, d(GCGAATTCGC)2, obtained by restrained molecular dynamics. Interproton distance and deoxyribose ring torsion angle restraints were deduced from homonuclear NOESY and DQF-COSY data, respectively. For both the control and alphaT duplexes, excellent global convergence was observed from two different (A- and B-) starting models. The final average structures of the two duplexes are highly homologous, and overall possess the traits characteristic of right-handed B-DNA duplexes. However, localized differences between the two structures stem from the enhanced conformational exchange in the deoxyribose ring of the cytidine following the 5'-5' linkage, the C3'- exo pseudorotation phase angle of the alpha-nucleotide, and unusual backbone torsions in the 3'-3' and 5'-5' phosphodiester linkages. The structural data reported here are relevant to the design of antisense therapeutics comprised of these modifications.
The line below this paragraph, {{ABSTRACT_PUBMED_9837995}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_9837995}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BX5 OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BX5 OCA].  


==Reference==
==Reference==
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[[Category: Alpha anomeric]]
[[Category: Alpha anomeric]]
[[Category: Polarity reversal]]
[[Category: Polarity reversal]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:04:02 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:51:29 2008''

Revision as of 16:51, 30 June 2008

File:1bx5.png

Template:STRUCTURE 1bx5

NMR SOLUTION STRUCTURE OF [D(GCGAAT-3'-3'-ALPHAT-5'-5'-CGC)2]

Template:ABSTRACT PUBMED 9837995

About this Structure

Full experimental information is available from OCA.

Reference

NMR solution structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its unmodified control., Aramini JM, Mujeeb A, Germann MW, Nucleic Acids Res. 1998 Dec 15;26(24):5644-54. PMID:9837995

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