1c5e: Difference between revisions

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[[Image:1c5e.gif|left|200px]]
{{Seed}}
[[Image:1c5e.png|left|200px]]


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{{STRUCTURE_1c5e|  PDB=1c5e  |  SCENE=  }}  
{{STRUCTURE_1c5e|  PDB=1c5e  |  SCENE=  }}  


'''BACTERIOPHAGE LAMBDA HEAD PROTEIN D'''
===BACTERIOPHAGE LAMBDA HEAD PROTEIN D===




==Overview==
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The crystal structure of gpD, the capsid-stabilizing protein of bacteriophage lambda, was solved at 1.1 A resolution. Data were obtained from twinned crystals in space group P21 and refined with anisotropic temperature factors to an R-factor of 0.098 (Rfree = 0. 132). GpD (109 residues) has a novel fold with an unusually low content of regular secondary structure. Noncrystallographic trimers with substantial intersubunit interfaces were observed. The C-termini are well ordered and located on one side of the trimer, relatively far from its three-fold axis. The N-termini are disordered up to Ser 15, which is close to the three-fold axis and on the same side as the C-termini. A density map of the icosahedral viral capsid at 15 A resolution, obtained by cryo-electron microscopy and image reconstruction, reveals gpD trimers, seemingly indistinguishable from the ones seen in the crystals, at all three-fold sites. The map further reveals that the side of the trimer that binds to the capsid is the side on which both termini reside. Despite this orientation of the gpD trimer, fusion proteins connected by linker peptides to either terminus bind to the capsid, allowing protein and peptide display.
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{{ABSTRACT_PUBMED_10700283}}


==About this Structure==
==About this Structure==
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[[Category: Protein crystal structure]]
[[Category: Protein crystal structure]]
[[Category: Virus assembly]]
[[Category: Virus assembly]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:20:58 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:14:09 2008''

Revision as of 17:14, 30 June 2008

File:1c5e.png

Template:STRUCTURE 1c5e

BACTERIOPHAGE LAMBDA HEAD PROTEIN D

Template:ABSTRACT PUBMED 10700283

About this Structure

1C5E is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.

Reference

Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD., Yang F, Forrer P, Dauter Z, Conway JF, Cheng N, Cerritelli ME, Steven AC, Pluckthun A, Wlodawer A, Nat Struct Biol. 2000 Mar;7(3):230-7. PMID:10700283

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