1c7v: Difference between revisions

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[[Image:1c7v.gif|left|200px]]
{{Seed}}
[[Image:1c7v.png|left|200px]]


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{{STRUCTURE_1c7v|  PDB=1c7v  |  SCENE=  }}  
{{STRUCTURE_1c7v|  PDB=1c7v  |  SCENE=  }}  


'''NMR SOLUTION STRUCTURE OF THE CALCIUM-BOUND C-TERMINAL DOMAIN (W81-S161) OF CALCIUM VECTOR PROTEIN FROM AMPHIOXUS'''
===NMR SOLUTION STRUCTURE OF THE CALCIUM-BOUND C-TERMINAL DOMAIN (W81-S161) OF CALCIUM VECTOR PROTEIN FROM AMPHIOXUS===




==Overview==
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Calcium vector protein (CaVP) from amphioxus is a two-domain, calcium-binding protein (18.3 kDa) of the calmodulin superfamily. Only two of the four EF-hand motifs (sites III and IV) have a significant binding affinity for calcium ions. We determined the solution structure of the domain containing these active sites (C-CaVP: W81-S161), in the Ca(2+)-saturated state, using NMR spectroscopy and restrained molecular dynamics. The tertiary structure is similar to other Ca(2+)-binding domains containing a pair of EF-hand motifs. The apo state has spectroscopic and thermodynamic characteristics of a molten globule, with conserved secondary structure but highly fluctuating tertiary organization. Titration of C-CaVP with Ca(2+) revealed a stepwise ion binding, with a stable equilibrium intermediate in which only site III binds a calcium ion. Despite a highly fluctuating structure of the free site IV, the calcium-bound site III has a persistent structure, with similar secondary elements but different interhelix angle and hydrophobic packing relative to the fully calcium-saturated state.
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{{ABSTRACT_PUBMED_10891072}}


==About this Structure==
==About this Structure==
1C7V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C7V OCA].  
1C7V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C7V OCA].  


==Reference==
==Reference==
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[[Category: Ef-hand family]]
[[Category: Ef-hand family]]
[[Category: Nmr]]
[[Category: Nmr]]
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