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| {{STRUCTURE_1cc0| PDB=1cc0 | SCENE= }} | | {{STRUCTURE_1cc0| PDB=1cc0 | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX'''
| | ===CRYSTAL STRUCTURE OF THE RHOA.GDP-RHOGDI COMPLEX=== |
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| ==Overview==
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| Like all Rho (Ras homology) GTPases, RhoA functions as a molecular switch in cell signaling, alternating between GTP- and GDP-bound states, with its biologically inactive GDP-bound form maintained as a cytosolic complex with RhoGDI (guanine nucleotide-exchange inhibitor). The crystal structures of RhoA-GDP and of the C-terminal immunoglobulin-like domain of RhoGDI (residues 67-203) are known, but the mechanism by which the two proteins interact is not known. The functional human RhoA-RhoGDI complex has been expressed in yeast and crystallized (P6(5)22, unit-cell parameters a = b = 139, c = 253 A, two complexes in the asymmetric unit). Although diffraction from these crystals extends to 3.5 A and is highly anisotropic, the experimentally phased (MAD plus MIR) electron-density map was adequate to reveal the mutual disposition of the two molecules. The result was validated by molecular-replacement calculations when data were corrected for anisotropy. Furthermore, the N-terminus of RhoGDI (the region involved in inhibition of nucleotide exchange) can be identified in the electron-density map: it is bound to the switch I and switch II regions of RhoA, occluding an epitope which binds Dbl-like nucleotide-exchange factors. The entrance of the hydrophobic pocket of RhoGDI is 25 A from the last residue in the RhoA model, with its C-terminus oriented to accommodate the geranylgeranyl group without conformational change in RhoA.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10489445}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10489445 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10489445}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: G-protein]] | | [[Category: G-protein]] |
| [[Category: Rho gtpase]] | | [[Category: Rho gtpase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:33:57 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:32:03 2008'' |
