1cgh: Difference between revisions

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[[Image:1cgh.gif|left|200px]]
{{Seed}}
[[Image:1cgh.png|left|200px]]


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{{STRUCTURE_1cgh|  PDB=1cgh  |  SCENE=  }}  
{{STRUCTURE_1cgh|  PDB=1cgh  |  SCENE=  }}  


'''HUMAN CATHEPSIN G'''
===HUMAN CATHEPSIN G===




==Overview==
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The crystal structure of human neutrophil cathepsin G, complexed with the peptidyl phosphonate inhibitor Suc-Val-Pro-PheP-(OPh)2, has been determined to a resolution of 1.8 A using Patterson search techniques. The cathepsin G structure shows the polypeptide fold characteristic of trypsin-like serine proteinases and is especially similar to rat mast cell proteinase II. Unique to cathepsin G, however, is the presence of Glu226 (chymotrypsinogen numbering), which is situated at the bottom of the S1 specificity pocket, dividing it into two compartments. For this reason, the benzyl side chain of the inhibitor PheP residue does not fully occupy the pocket but is, instead, located at its entrance. Its positively charged equatorial edge is involved in a favourable electrostatic interaction with the negatively charged carboxylate group of Glu226. Arrangement of this Glu226 carboxylate would also allow accommodation of a Lys side chain in this S1 pocket, in agreement with the recently observed cathepsin G preference for Lys and Phe at P1. The cathepsin G complex with the covalently bound phosphonate inhibitor mimics a tetrahedral substrate intermediate. A comparison of the Arg surface distributions of cathepsin G, leukocyte elastase and rat mast cell protease II shows no simple common recognition pattern for a mannose-6-phosphate receptor-independent targeting mechanism for sorting of these granular proteinases.
The line below this paragraph, {{ABSTRACT_PUBMED_8896442}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 8896442 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8896442}}


==About this Structure==
==About this Structure==
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[[Category: Serine protease]]
[[Category: Serine protease]]
[[Category: Specificity]]
[[Category: Specificity]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:42:36 2008''
 
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Revision as of 17:43, 30 June 2008

File:1cgh.png

Template:STRUCTURE 1cgh

HUMAN CATHEPSIN G

Template:ABSTRACT PUBMED 8896442

About this Structure

1CGH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The 1.8 A crystal structure of human cathepsin G in complex with Suc-Val-Pro-PheP-(OPh)2: a Janus-faced proteinase with two opposite specificities., Hof P, Mayr I, Huber R, Korzus E, Potempa J, Travis J, Powers JC, Bode W, EMBO J. 1996 Oct 15;15(20):5481-91. PMID:8896442

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