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| {{STRUCTURE_1chd| PDB=1chd | SCENE= }} | | {{STRUCTURE_1chd| PDB=1chd | SCENE= }} |
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| '''CHEB METHYLESTERASE DOMAIN'''
| | ===CHEB METHYLESTERASE DOMAIN=== |
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| ==Overview==
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| Signaling activity of bacterial chemotaxis transmembrane receptors is modulated by reversible covalent modification of specific receptor glutamate residues. The level of receptor methylation results from the activities of a specific S-adenosylmethionine-dependent methyltransferase, CheR, and the CheB methylesterase, which catalyzes hydrolysis of receptor glutamine or methylglutamate side-chains to glutamic acid. The CheB methylesterase belongs to a large family of response regulator proteins in which N-terminal regulatory domains control the activities of C-terminal effector domains. The crystal structure of the catalytic domain of the Salmonella typhimurium CheB methylesterase has been determined at 1.75 A resolution. The domain has a modified, doubly wound alpha/beta fold in which one of the helices is replaced by an anti-parallel beta-hairpin. Previous biochemical and mutagenesis data, suggest that the methylester hydrolysis catalyzed by CheB proceeds through a mechanism involving a serine nucleophile. The methylesterase active site is tentatively identified as a cleft at the C-terminal edge of the beta-sheet containing residues Ser164, His190 and Asp286. The three-dimensional fold, and the arrangement of residues within the catalytic triad distinguishes the CheB methylesterase from any previously described serine protease or serine hydrolase.
| | The line below this paragraph, {{ABSTRACT_PUBMED_7608974}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 7608974 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_7608974}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Chemotaxis protein]] | | [[Category: Chemotaxis protein]] |
| [[Category: Serine hydrolase]] | | [[Category: Serine hydrolase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:44:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:44:58 2008'' |
