1chk: Difference between revisions

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[[Image:1chk.gif|left|200px]]
{{Seed}}
[[Image:1chk.png|left|200px]]


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{{STRUCTURE_1chk|  PDB=1chk  |  SCENE=  }}  
{{STRUCTURE_1chk|  PDB=1chk  |  SCENE=  }}  


'''STREPTOMYCES N174 CHITOSANASE PH5.5 298K'''
===STREPTOMYCES N174 CHITOSANASE PH5.5 298K===




==Overview==
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We report the 2.4 A X-ray crystal structure of a protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The structure was solved using phases acquired by SIRAS from a two-site methyl mercury derivative combined with solvent flattening and non-crystallographic two-fold symmetry averaging, and refined to an R-factor of 18.5%. The mostly alpha-helical fold reveals a structural core shared with several classes of lysozyme and barley endochitinase, in spite of a lack of shared sequence. Based on this structural similarity we postulate a putative active site, mechanism of action and mode of substrate recognition. It appears that Glu 22 acts as an acid and Asp 40 serves as a general base to activate a water molecule for an SN2 attack on the glycosidic bond. A series of amino-acid side chains and backbone carbonyl groups may bind the polycationic chitosan substrate in a deep electronegative binding cleft.
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{{ABSTRACT_PUBMED_8564542}}


==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: O-glycosyl]]
[[Category: O-glycosyl]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:44:18 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:45:18 2008''

Revision as of 17:45, 30 June 2008

File:1chk.png

Template:STRUCTURE 1chk

STREPTOMYCES N174 CHITOSANASE PH5.5 298K

Template:ABSTRACT PUBMED 8564542

About this Structure

1CHK is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.

Reference

X-ray structure of an anti-fungal chitosanase from streptomyces N174., Marcotte EM, Monzingo AF, Ernst SR, Brzezinski R, Robertus JD, Nat Struct Biol. 1996 Feb;3(2):155-62. PMID:8564542

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