|
|
| Line 1: |
Line 1: |
| [[Image:1ck4.gif|left|200px]] | | {{Seed}} |
| | [[Image:1ck4.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1ck4| PDB=1ck4 | SCENE= }} | | {{STRUCTURE_1ck4| PDB=1ck4 | SCENE= }} |
|
| |
|
| '''CRYSTAL STRUCTURE OF RAT A1B1 INTEGRIN I-DOMAIN.'''
| | ===CRYSTAL STRUCTURE OF RAT A1B1 INTEGRIN I-DOMAIN.=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| The alpha1beta1 integrin is a major cell surface receptor for collagen. Ligand binding is mediated, in part, through a 200 amino acid inserted 'I'-domain contained in the extracellular part of the integrin alpha chain. Integrin I-domains contain a divalent cation binding (MIDAS) site and require cations to interact with integrin ligands. We have determined the crystal structure of recombinant I-domain from the rat alpha1beta1 integrin at 2.2 A resolution in the absence of divalent cations. The alpha1 I-domain adopts the dinucleotide binding fold that is characteristic of all I-domain structures that have been solved to date and has a structure very similar to that of the closely related alpha2beta1 I-domain which also mediates collagen binding. A unique feature of the alpha1 I-domain crystal structure is that the MIDAS site is occupied by an arginine side chain from another I-domain molecule in the crystal, in place of a metal ion. This interaction supports a proposed model for ligand-induced displacement of metal ions. Circular dichroism spectra determined in the presence of Ca2+, Mg2+ and Mn2+ indicate that no changes in the structure of the I-domain occur upon metal ion binding in solution. Metal ion binding induces small changes in UV absorption spectra, indicating a change in the polarity of the MIDAS site environment. | | The line below this paragraph, {{ABSTRACT_PUBMED_10386626}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10386626 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_10386626}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 32: |
Line 36: |
| [[Category: I-domain]] | | [[Category: I-domain]] |
| [[Category: Metal binding]] | | [[Category: Metal binding]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:49:17 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:51:28 2008'' |
