1cx6: Difference between revisions

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[[Image:1cx6.~~gif~~|left|200px]]

[[Image:1cx6.png|left|200px]]

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~~'''~~T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE~~'''~~

===T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE===

~~==Overview==~~

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The ~~availability of a series of phage T4 lysozymes with up~~ to ~~14 methionine residues incorporated within~~ the ~~protein has made~~ it ~~possible to systematically compare the effect~~ on ~~protein stability of selenomethionine relative to methionine~~. ~~Wild-type lysozyme contains two fully buried methionine residues plus three more on the surface~~. ~~The substitution of these methionine residues with selenomethionine slightly stabilizes the protein. As more and more methionine residues are substituted into the protein~~, ~~there~~ is ~~a progressive loss of stability. This is, however, increasingly offset in~~ the selenomethionine variants, ultimately resulting in a differential increase in melting temperature of about 7 degrees C. This increase, corresponding to about 0.25 kcal/mol per substitution, is in reasonable agreement with the difference in the solvent transfer free energy between the two amino acids.

The line below this paragraph, {{ABSTRACT_PUBMED_10556025}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 10556025 is the PubMed ID number.

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==About this Structure==

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[[Category: Selenomethionine core mutant]]

[[Category: T4 lysozyme]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May 2 13~~:12:39 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:41:37 2008''

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-[[Image:1cx6.gif|left|200px]]
+{{Seed}}
+[[Image:1cx6.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_1cx6|  PDB=1cx6  |  SCENE=  }}
-'''T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE'''
+===T4 LYSOZYME SUBSTITUTED WITH SELENOMETHIONINE===
-==Overview==
+<!--
-The availability of a series of phage T4 lysozymes with up to 14 methionine residues incorporated within the protein has made it possible to systematically compare the effect on protein stability of selenomethionine relative to methionine. Wild-type lysozyme contains two fully buried methionine residues plus three more on the surface. The substitution of these methionine residues with selenomethionine slightly stabilizes the protein. As more and more methionine residues are substituted into the protein, there is a progressive loss of stability. This is, however, increasingly offset in the selenomethionine variants, ultimately resulting in a differential increase in melting temperature of about 7 degrees C. This increase, corresponding to about 0.25 kcal/mol per substitution, is in reasonable agreement with the difference in the solvent transfer free energy between the two amino acids.
+The line below this paragraph, {{ABSTRACT_PUBMED_10556025}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10556025 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_10556025}}
 ==About this Structure==
@@ Line 30: / Line 34: @@
 [[Category: Selenomethionine core mutant]]
 [[Category: T4 lysozyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:12:39 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:41:37 2008''