1d3c: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:1d3c.gif|left|200px]]
{{Seed}}
[[Image:1d3c.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1d3c|  PDB=1d3c  |  SCENE=  }}  
{{STRUCTURE_1d3c|  PDB=1d3c  |  SCENE=  }}  


'''MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN'''
===MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN===




==Overview==
<!--
The enzyme cyclodextrin glycosyltransferase is closely related to alpha-amylases but has the unique ability to produce cyclodextrins (circular alpha(1--&gt;4)-linked glucoses) from starch. To characterize this specificity we determined a 1.8-A structure of an E257Q/D229N mutant cyclodextrin glycosyltransferase in complex with its product gamma-cyclodextrin, which reveals for the first time how cyclodextrin is competently bound. Across subsites -2, -1, and +1, the cyclodextrin ring binds in a twisted mode similar to linear sugars, giving rise to deformation of its circular symmetry. At subsites -3 and +2, the cyclodextrin binds in a manner different from linear sugars. Sequence comparisons and site-directed mutagenesis experiments support the conclusion that subsites -3 and +2 confer the cyclization activity in addition to subsite -6 and Tyr-195. On this basis, a role of the individual residues during the cyclization reaction cycle is proposed.
The line below this paragraph, {{ABSTRACT_PUBMED_10574960}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10574960 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_10574960}}


==About this Structure==
==About this Structure==
Line 35: Line 39:
[[Category: Product complex]]
[[Category: Product complex]]
[[Category: Transglycosylation]]
[[Category: Transglycosylation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:24:13 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:17:28 2008''

Revision as of 19:17, 30 June 2008

File:1d3c.png

Template:STRUCTURE 1d3c

MICHAELIS COMPLEX OF BACILLUS CIRCULANS STRAIN 251 CYCLODEXTRIN GLYCOSYLTRANSFERASE WITH GAMMA-CYCLODEXTRIN

Template:ABSTRACT PUBMED 10574960

About this Structure

1D3C is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

Reference

The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a gamma-cyclodextrin-CGTase complex at 1.8-A resolution., Uitdehaag JC, Kalk KH, van Der Veen BA, Dijkhuizen L, Dijkstra BW, J Biol Chem. 1999 Dec 3;274(49):34868-76. PMID:10574960

Page seeded by OCA on Mon Jun 30 22:17:28 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1d3c&oldid=585546"