1d75: Difference between revisions

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[[Image:1d75.gif|left|200px]]
{{Seed}}
[[Image:1d75.png|left|200px]]


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{{STRUCTURE_1d75|  PDB=1d75  |  SCENE=  }}  
{{STRUCTURE_1d75|  PDB=1d75  |  SCENE=  }}  


'''CONFORMATION OF THE GUANINE.8-OXOADENINE BASE PAIRS IN THE CRYSTAL STRUCTURE OF D(CGCGAATT(O8A)GCG)'''
===CONFORMATION OF THE GUANINE.8-OXOADENINE BASE PAIRS IN THE CRYSTAL STRUCTURE OF D(CGCGAATT(O8A)GCG)===




==Overview==
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The structure of the synthetic deoxydodecamer d(CGCGAATT(O8A)GCG)2 (O8A = 8-oxoadenine) has been determined by single-crystal X-ray diffraction techniques. The oligonucleotide crystallizes in the orthorhombic space group P2(1)2(1)2(1) with cell dimensions of a = 25.48 A, b = 41.84 A, and c = 64.91 A. The refinement has converged with an R-factor of 0.151 for 1119 reflections in the resolution range 8.0-2.25 A. Sixty-seven solvent molecules were located during the course of the refinement. The B-DNA helix consists of ten Watson-Crick base pairs and two guanine-8-oxoadenine (G.O8A) base pairs. In order to achieve hydrogen-bonding complementarity between the two bases, an unusual G(anti).O8A-(syn) wobble conformation is adopted. It is proposed that the G.O8A mispairs are held together by a network of four interbase hydrogen bonds which are the result of the formation of two reverse three-center hydrogen-bonding systems. These involve one carbonyl oxygen lone pair interacting with two hydrogen atoms. In a departure from previous observations of the characteristics of purine-purine anti-syn base pairs, lambda 1 and lambda 2, the angles between the glycosidic bonds and the C1'-C1' vector, are symmetric. A reassessment of the other purine-purine mispairs suggests that similar three-center hydrogen bonds may occur and make a contribution to stabilizing other base pairings.
The line below this paragraph, {{ABSTRACT_PUBMED_1390625}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_1390625}}


==About this Structure==
==About this Structure==
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[[Category: Mismatched]]
[[Category: Mismatched]]
[[Category: Modified]]
[[Category: Modified]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:31:19 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:34:32 2008''

Revision as of 19:34, 30 June 2008

File:1d75.png

Template:STRUCTURE 1d75

CONFORMATION OF THE GUANINE.8-OXOADENINE BASE PAIRS IN THE CRYSTAL STRUCTURE OF D(CGCGAATT(O8A)GCG)

Template:ABSTRACT PUBMED 1390625

About this Structure

Full crystallographic information is available from OCA.

Reference

Conformation of guanine-8-oxoadenine base pairs in the crystal structure of d(CGCGAATT(O8A)GCG)., Leonard GA, Guy A, Brown T, Teoule R, Hunter WN, Biochemistry. 1992 Sep 15;31(36):8415-20. PMID:1390625

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