1dbi: Difference between revisions

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[[Image:1dbi.gif|left|200px]]
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{{STRUCTURE_1dbi|  PDB=1dbi  |  SCENE=  }}  
{{STRUCTURE_1dbi|  PDB=1dbi  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF A THERMOSTABLE SERINE PROTEASE'''
===CRYSTAL STRUCTURE OF A THERMOSTABLE SERINE PROTEASE===




==Overview==
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Proteins of the subtilisin superfamily (subtilases) are widely distributed through many living species, where they perform a variety of processing functions. They are also used extensively in industry. In many of these enzymes, bound calcium ions play a key role in protecting against autolysis and thermal denaturation. We have determined the crystal structure of a highly thermostable protease from Bacillus sp. Ak.1 that is strongly stabilized by calcium. The crystal structure, determined at 1.8 A resolution (R=0. 182, Rfree=0.247), reveals the presence of four bound cations, three Ca(2+) and one Na(+). Two of the Ca(2+) binding sites, Ca-1 and Ca-2, correspond to sites also found in thermitase and the mesophilic subtilisins. The third calcium ion, however, is at a novel site that is created by two key amino acid substitutions near Ca-1, and has not been observed in any other subtilase. This site, acting cooperatively with Ca-1, appears to give substantially enhanced thermostability, compared with thermitase. Comparisons with the mesophilic subtilisins also point to the importance of aromatic clusters, reduced hydrophobic surface and constrained N and C termini in enhancing the thermostability of thermitase and Ak.1 protease. The Ak.1 protease also contains an unusual Cys-X-Cys disulfide bridge that modifies the active site cleft geometry.
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{{ABSTRACT_PUBMED_10588904}}


==About this Structure==
==About this Structure==
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[[Category: Toogood, H S.]]
[[Category: Toogood, H S.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:46:50 2008''

Revision as of 19:46, 30 June 2008

File:1dbi.png

Template:STRUCTURE 1dbi

CRYSTAL STRUCTURE OF A THERMOSTABLE SERINE PROTEASE

Template:ABSTRACT PUBMED 10588904

About this Structure

1DBI is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

Calcium-mediated thermostability in the subtilisin superfamily: the crystal structure of Bacillus Ak.1 protease at 1.8 A resolution., Smith CA, Toogood HS, Baker HM, Daniel RM, Baker EN, J Mol Biol. 1999 Dec 10;294(4):1027-40. PMID:10588904

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