|
|
| Line 1: |
Line 1: |
| [[Image:1dcs.gif|left|200px]] | | {{Seed}} |
| | [[Image:1dcs.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1dcs| PDB=1dcs | SCENE= }} | | {{STRUCTURE_1dcs| PDB=1dcs | SCENE= }} |
|
| |
|
| '''DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS'''
| | ===DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| Penicillins and cephalosporins are among the most widely used therapeutic agents. These antibiotics are produced from fermentation-derived materials as their chemical synthesis is not commercially viable. Unconventional steps in their biosynthesis are catalysed by Fe(II)-dependent oxidases/oxygenases; isopenicillin N synthase (IPNS) creates in one step the bicyclic nucleus of penicillins, and deacetoxycephalosporin C synthase (DAOCS) catalyses the expansion of the penicillin nucleus into the nucleus of cephalosporins. Both enzymes use dioxygen-derived ferryl intermediates in catalysis but, in contrast to IPNS, the ferryl form of DAOCS is produced by the oxidative splitting of a co-substrate, 2-oxoglutarate (alpha-ketoglutarate). This route of controlled ferryl formation and reaction is common to many mononuclear ferrous enzymes, which participate in a broader range of reactions than their well-characterized counterparts, the haem enzymes. Here we report the first crystal structure of a 2-oxoacid-dependent oxygenase. High-resolution structures for apo-DAOCS, the enzyme complexed with Fe(II), and with Fe(II) and 2-oxoglutarate, were obtained from merohedrally twinned crystals. Using a model based on these structures, we propose a mechanism for ferryl formation.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9723623}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9723623 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_9723623}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 41: |
Line 45: |
| [[Category: Merohedral twinning]] | | [[Category: Merohedral twinning]] |
| [[Category: Oxidoreductase]] | | [[Category: Oxidoreductase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:42:12 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:50:10 2008'' |
