1dm4: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,097 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,097 edits
No edit summary
Line 1: Line 1:
[[Image:1dm4.gif|left|200px]]
{{Seed}}
[[Image:1dm4.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1dm4|  PDB=1dm4  |  SCENE=  }}  
{{STRUCTURE_1dm4|  PDB=1dm4  |  SCENE=  }}  


'''SER195ALA MUTANT OF HUMAN THROMBIN COMPLEXED WITH FIBRINOPEPTIDE A (7-16)'''
===SER195ALA MUTANT OF HUMAN THROMBIN COMPLEXED WITH FIBRINOPEPTIDE A (7-16)===




==Overview==
<!--  
The Ser195Ala mutant of human alpha-thrombin was complexed with fibrinopeptide A(7-22) (FPA) in an effort to describe the (P1'-P6') post-cleavage binding subsites of the fibrinogen-recognition exosite and define more clearly the nature of the Michaelis complex and the scissile peptide bond bound at the catalytic site. The thrombin mutant, however, has residual catalytic activity and proteolysis occurred at the Arg16-Gly17 bond. Thus, the structure of the thrombin complex determined was that of FPA(7-16) bound at the active site, which is very similar to the ternary FPA(7-16)cmk-human thrombin-hirugen complex (r.m.s.d. approximately 0.4 A; Stubbs et al. , 1992). It is further shown by subsidiary experiments that the cleavage is the result of residual catalytic activity of the altered catalytic machinery.
The line below this paragraph, {{ABSTRACT_PUBMED_10739913}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 10739913 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_10739913}}


==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Mutant thrombin]]
[[Category: Mutant thrombin]]
[[Category: Residual catalytic activity]]
[[Category: Residual catalytic activity]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:00:32 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:16:47 2008''
Retrieved from "https://proteopedia.org/1dm4"