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| [[Image:1dn0.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1dn0| PDB=1dn0 | SCENE= }} | | {{STRUCTURE_1dn0| PDB=1dn0 | SCENE= }} |
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| '''STRUCTURE OF THE FAB FRAGMENT FROM A HUMAN IGM COLD AGGLUTININ'''
| | ===STRUCTURE OF THE FAB FRAGMENT FROM A HUMAN IGM COLD AGGLUTININ=== |
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| ==Overview==
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| Cold agglutinins (CAs) are IgM autoantibodies characterized by their ability to agglutinate in vitro RBC at low temperatures. These autoantibodies cause hemolytic anemia in patients with CA disease. Many diverse Ags are recognized by CAs, most frequently those belonging to the I/i system. These are oligosaccharides composed of repeated units of N:-acetyllactosamine, expressed on RBC. The three-dimensional structure of the Fab of KAU, a human monoclonal IgM CA with anti-I activity, was determined. The KAU combining site shows an extended cavity and a neighboring pocket. Residues from the hypervariable loops V(H)CDR3, V(L)CDR1, and V(L)CDR3 form the cavity, whereas the small pocket is defined essentially by residues from the hypervariable loops V(H)CDR1 and V(H)CDR2. This fact could explain the V(H)4-34 germline gene restriction among CA. The KAU combining site topography is consistent with one that binds a polysaccharide. The combining site overall dimensions are 15 A wide and 24 A long. Conservation of key binding site residues among anti-I/i CAs indicates that this is a common feature of this family of autoantibodies. We also describe the first high resolution structure of the human IgM C(H)1:C(L) domain. The structural analysis shows that the C(H)1-C(L) interface is mainly conserved during the isotype switch process from IgM to IgG1.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11086081}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11086081 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11086081}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Human]] | | [[Category: Human]] |
| [[Category: Igm]] | | [[Category: Igm]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:02:31 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:19:24 2008'' |
