1dne: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,329,557 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,329,557 edits
No edit summary
Line 1: Line 1:
[[Image:1dne.gif|left|200px]]
{{Seed}}
[[Image:1dne.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1dne|  PDB=1dne  |  SCENE=  }}  
{{STRUCTURE_1dne|  PDB=1dne  |  SCENE=  }}  


'''MOLECULAR STRUCTURE OF THE NETROPSIN-D(CGCGATATCGCG) COMPLEX: DNA CONFORMATION IN AN ALTERNATING AT SEGMENT; CONFORMATION 2'''
===MOLECULAR STRUCTURE OF THE NETROPSIN-D(CGCGATATCGCG) COMPLEX: DNA CONFORMATION IN AN ALTERNATING AT SEGMENT; CONFORMATION 2===




==Overview==
<!--  
The molecular structure of the complex between a minor groove binding drug (netropsin) and the DNA dodecamer d(CGCGATATCGCG) has been solved and refined by single-crystal X-ray diffraction analysis to a final R factor of 20.0% to 2.4-A resolution. The crystal is similar to that of the other related dodecamers with unit cell dimensions of a = 25.48 A, b = 41.26 A, and c = 66.88 A in the space group P2(1)2(1)2(1). In the complex, netropsin binds to the central ATAT tetranucleotide segment in the narrow minor groove of the dodecamer B-DNA double helix as expected. However, in the structural refinement the drug is found to fit the electron density in two orientations equally well, suggesting the disordered model. This agrees with the results from solution studies (chemical footprinting and NMR) of the interactions between minor groove binding drugs (e.g., netropsin and distamycin A) and DNA. The stabilizing forces between drug and DNA are provided by a combination of ionic, van der Waals, and hydrogen-bonding interactions. No bifurcated hydrogen bond is found between netropsin and DNA in this complex due to the unique dispositions of the hydrogen-bond acceptors (N3 of adenine and O2 of thymine) on the floor of the DNA minor groove. Two of the four AT base pairs in the ATAT stretch have low propeller twist angles, even though the DNA has a narrow minor groove. Alternating helical twist angles are observed in the ATAT stretch with lower twist in the ApT steps than in the TpA step.
The line below this paragraph, {{ABSTRACT_PUBMED_2539859}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 2539859 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_2539859}}


==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Complexed with drug]]
[[Category: Complexed with drug]]
[[Category: Double helix]]
[[Category: Double helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:03:03 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:20:01 2008''

Revision as of 20:20, 30 June 2008

File:1dne.png

Template:STRUCTURE 1dne

MOLECULAR STRUCTURE OF THE NETROPSIN-D(CGCGATATCGCG) COMPLEX: DNA CONFORMATION IN AN ALTERNATING AT SEGMENT; CONFORMATION 2

Template:ABSTRACT PUBMED 2539859

About this Structure

Full crystallographic information is available from OCA.

Reference

Molecular structure of the netropsin-d(CGCGATATCGCG) complex: DNA conformation in an alternating AT segment., Coll M, Aymami J, van der Marel GA, van Boom JH, Rich A, Wang AH, Biochemistry. 1989 Jan 10;28(1):310-20. PMID:2539859

Page seeded by OCA on Mon Jun 30 23:20:01 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1dne&oldid=588223"