1dzb: Difference between revisions

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[[Image:1dzb.gif|left|200px]]
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[[Image:1dzb.png|left|200px]]


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{{STRUCTURE_1dzb|  PDB=1dzb  |  SCENE=  }}  
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'''CRYSTAL STRUCTURE OF PHAGE LIBRARY-DERIVED SINGLE-CHAIN FV FRAGMENT 1F9 IN COMPLEX WITH TURKEY EGG-WHITE LYSOZYME'''
===CRYSTAL STRUCTURE OF PHAGE LIBRARY-DERIVED SINGLE-CHAIN FV FRAGMENT 1F9 IN COMPLEX WITH TURKEY EGG-WHITE LYSOZYME===




==Overview==
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The three-dimensional structure of the single-chain Fv fragment 1F9 in complex with turkey egg-white lysozyme (TEL) has been determined to a nominal resolution of 2.0 A by X-ray diffraction. The scFv fragment 1F9 was derived from phage-display libraries in two steps and binds both hen and turkey egg-white lysozyme, although the level of binding affinity is two orders of magnitude greater for the turkey lysozyme. The comparison of the crystal structure with a model of the single-chain Fv fragment 1F9 in complex with hen egg-white lysozyme (HEL) reveals that in the latter a clash between Asp101 in lysozyme and Trp98 of the complementarity determining region H3 of the heavy chain variable domain occurs. This is the only explanation apparent from the crystal structure for the better binding of TEL compared to HEL.The binding site topology on the paratope is not simply a planar surface as is usually found in antibody-protein interfaces, but includes a cleft between the light chain variable domain and heavy chain variable domain large enough to accommodate a loop from the lysozyme. The scFv fragment 1F9 recognizes an epitope on TEL that differs from the three antigenic determinants recognized in other known crystal structures of monoclonal antibodies in complex with lysozyme.
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{{ABSTRACT_PUBMED_10926506}}


==About this Structure==
==About this Structure==
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[[Category: Single-domain antibody]]
[[Category: Single-domain antibody]]
[[Category: Turkey egg-white lysozyme]]
[[Category: Turkey egg-white lysozyme]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 14:27:55 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:52:12 2008''

Revision as of 20:52, 30 June 2008

File:1dzb.png

Template:STRUCTURE 1dzb

CRYSTAL STRUCTURE OF PHAGE LIBRARY-DERIVED SINGLE-CHAIN FV FRAGMENT 1F9 IN COMPLEX WITH TURKEY EGG-WHITE LYSOZYME

Template:ABSTRACT PUBMED 10926506

About this Structure

1DZB is a Single protein structure of sequence from Meleagris gallopavo and Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of a phage library-derived single-chain Fv fragment complexed with turkey egg-white lysozyme at 2.0 A resolution., Ay J, Keitel T, Kuttner G, Wessner H, Scholz C, Hahn M, Hohne W, J Mol Biol. 2000 Aug 11;301(2):239-46. PMID:10926506

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