1e3r: Difference between revisions

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[[Image:1e3r.jpg|left|200px]]
{{Seed}}
[[Image:1e3r.png|left|200px]]


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{{STRUCTURE_1e3r|  PDB=1e3r  |  SCENE=  }}  
{{STRUCTURE_1e3r|  PDB=1e3r  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF KETOSTEROID ISOMERASE MUTANT D40N (D38N TI NUMBERING) FROM PSEUDOMONAS PUTIDA COMPLEXED WITH ANDROSTEN-3BETA-OL-17-ONE'''
===CRYSTAL STRUCTURE OF KETOSTEROID ISOMERASE MUTANT D40N (D38N TI NUMBERING) FROM PSEUDOMONAS PUTIDA COMPLEXED WITH ANDROSTEN-3BETA-OL-17-ONE===




==Overview==
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Delta(5)-3-Ketosteroid isomerase catalyzes cleavage and formation of a C-H bond at a diffusion-controlled limit. By determining the crystal structures of the enzyme in complex with each of three different inhibitors and by nuclear magnetic resonance (NMR) spectroscopic investigation, we evidenced the ionization of a hydroxyl group (pK(a) approximately 16.5) of an inhibitor, which forms a low barrier hydrogen bond (LBHB) with a catalytic residue Tyr(14) (pK(a) approximately 11.5), and the protonation of the catalytic residue Asp(38) with pK(a) of approximately 4.5 at pH 6.7 in the interaction with a carboxylate group of an inhibitor. The perturbation of the pK(a) values in both cases arises from the formation of favorable interactions between inhibitors and catalytic residues. The results indicate that the pK(a) difference between catalytic residue and substrate can be significantly reduced in the active site environment as a result of the formation of energetically favorable interactions during the course of enzyme reactions. The reduction in the pK(a) difference should facilitate the abstraction of a proton and thereby eliminate a large fraction of activation energy in general acid/base enzyme reactions. The pK(a) perturbation provides a mechanistic ground for the fast reactivity of many enzymes and for the understanding of how some enzymes are able to extract a proton from a C-H group with a pK(a) value as high as approximately 30.
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{{ABSTRACT_PUBMED_11007792}}


==About this Structure==
==About this Structure==
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[[Category: Ksi ketosteroied isomerase]]
[[Category: Ksi ketosteroied isomerase]]
[[Category: Lbhb]]
[[Category: Lbhb]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:37:41 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:06:04 2008''

Revision as of 21:06, 30 June 2008

File:1e3r.png

Template:STRUCTURE 1e3r

CRYSTAL STRUCTURE OF KETOSTEROID ISOMERASE MUTANT D40N (D38N TI NUMBERING) FROM PSEUDOMONAS PUTIDA COMPLEXED WITH ANDROSTEN-3BETA-OL-17-ONE

Template:ABSTRACT PUBMED 11007792

About this Structure

1E3R is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Detection of large pKa perturbations of an inhibitor and a catalytic group at an enzyme active site, a mechanistic basis for catalytic power of many enzymes., Ha NC, Kim MS, Lee W, Choi KY, Oh BH, J Biol Chem. 2000 Dec 29;275(52):41100-6. PMID:11007792

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