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| [[Image:1e5s.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1e5s| PDB=1e5s | SCENE= }} | | {{STRUCTURE_1e5s| PDB=1e5s | SCENE= }} |
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| '''PROLINE 3-HYDROXYLASE (TYPE II)-IRON FORM'''
| | ===PROLINE 3-HYDROXYLASE (TYPE II)-IRON FORM=== |
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| ==Overview==
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| Iron (II)/2-oxoglutarate (2-OG)-dependent oxygenases catalyse oxidative reactions in a range of metabolic processes including the hydroxylation of proline and lysine residues during the post-translational modification of collagen. 2-OG oxygenases commonly require ascorbate for full activity. In the vitamin C deficient disease, scurvy, reduced activity of 2-OG oxygenases results in impaired formation of collagen. Here we report the crystal structure of bacterial proline 3-hydroxylase from Streptomyces sp., an enzyme which hydroxylates proline at position 3, the first of a 2-OG oxygenase catalysing oxidation of a free alpha-amino acid. Structures were obtained for the enzyme in the absence of iron (to 2.3A resolution, R=20.2%, Rfree=25.3%) and that complexed to iron (II) (to 2.4A resolution, R=19.8%, Rfree=22.6%). The structure contains conserved motifs present in other 2-OG oxygenases including a 'jelly roll' beta strand core and residues binding iron and 2-oxoglutarate, consistent with divergent evolution within the extended family. The structure differs significantly from many other 2-OG oxygenases in possessing a discrete C-terminal helical domain. Analysis of the structure suggests a model for proline binding and a mechanism for uncoupling of proline and 2-OG turnover.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11737217}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11737217 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11737217}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Schofield, C J.]] | | [[Category: Schofield, C J.]] |
| [[Category: 2-oxoglutarate dependent oxygenase]] | | [[Category: 2-oxoglutarate dependent oxygenase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:42:00 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:11:44 2008'' |
