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| {{STRUCTURE_1e65| PDB=1e65 | SCENE= }} | | {{STRUCTURE_1e65| PDB=1e65 | SCENE= }} |
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| '''AZURIN FROM PSEUDOMONAS AERUGINOSA, APO FORM'''
| | ===AZURIN FROM PSEUDOMONAS AERUGINOSA, APO FORM=== |
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| ==Overview==
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| The 3D structure of apo-azurin from Pseudomonas aeruginosa has been determined at 1.85 A resolution. The crystal structure is composed of two different molecular forms of apo-azurin arranged as hetero-dimers in the tetramer of the asymmetric unit. Form 1 closely resembles the holo-protein lacking copper. Form 2 shows differences in the metal binding site region induced by the incorporation of a solvent molecule into this site. The positions of the copper ligands His46 and His117 are shifted by 0.6 A and 1.6 A. The His117 side chain adopts a position at the surface of the protein, thereby facilitating access to the copper site. The presence of two different molecular forms of apo-azurin in the crystal lattice may reflect an equilibrium between the two forms in solution. 1H-NMR spectra of apo-azurin recorded as a function of pH show that at high pH the line broadening of His35, His46 and His117 resonances is consistent with an interconversion between forms 1 and 2. At low pH, no broadening is observed. This may indicate that here the interconversion is fast on the NMR timescale. | | The line below this paragraph, {{ABSTRACT_PUBMED_1633865}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 1633865 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_1633865}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Messerschmidt, A.]] | | [[Category: Messerschmidt, A.]] |
| [[Category: Nar, H.]] | | [[Category: Nar, H.]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:42:52 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:12:48 2008'' |
