1e7n: Difference between revisions

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[[Image:1e7n.jpg|left|200px]]
{{Seed}}
[[Image:1e7n.png|left|200px]]


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{{STRUCTURE_1e7n|  PDB=1e7n  |  SCENE=  }}  
{{STRUCTURE_1e7n|  PDB=1e7n  |  SCENE=  }}  


'''THE N-TERMINAL DOMAIN OF BETA-B2-CRYSTALLIN RESEMBLES THE PUTATIVE ANCESTRAL HOMODIMER'''
===THE N-TERMINAL DOMAIN OF BETA-B2-CRYSTALLIN RESEMBLES THE PUTATIVE ANCESTRAL HOMODIMER===




==Overview==
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betagamma-crystallins from the eye lens are proteins consisting of two similar domains joined by a short linker. All three-dimensional structures of native proteins solved so far reveal similar pseudo-2-fold pairing of the domains reflecting their presumed ancient origin from a single-domain homodimer. However, studies of engineered single domains of members of the betagamma-crystallin superfamily have not revealed a prototype ancestral solution homodimer. Here we report the 2.35 A X-ray structure of the homodimer of the N-terminal domain of rat betaB2-crystallin (betaB2-N). The two identical domains pair in a symmetrical manner very similar to that observed in native betagamma-crystallins, where N and C-terminal domains (which share approximately 35% sequence identity) are related by a pseudo-2-fold axis. betaB2-N thus resembles the ancestral prototype of the betagamma-crystallin superfamily as it self-associates in solution to form a dimer with an essentially identical domain interface as that between the N and C domains in betagamma-crystallins, but without the benefit of a covalent linker. The structure provides further evidence for the role of two-domain pairing in stabilising the protomer fold. These results support the view that the betagamma-crystallin superfamily has evolved by a series of gene duplication and fusion events from a single-domain ancestor capable of forming homodimers.
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{{ABSTRACT_PUBMED_11090271}}


==About this Structure==
==About this Structure==
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[[Category: Eye lens]]
[[Category: Eye lens]]
[[Category: Protein structure]]
[[Category: Protein structure]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:45:58 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:17:05 2008''

Revision as of 21:17, 30 June 2008

File:1e7n.png

Template:STRUCTURE 1e7n

THE N-TERMINAL DOMAIN OF BETA-B2-CRYSTALLIN RESEMBLES THE PUTATIVE ANCESTRAL HOMODIMER

Template:ABSTRACT PUBMED 11090271

About this Structure

1E7N is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

The N-terminal domain of betaB2-crystallin resembles the putative ancestral homodimer., Clout NJ, Basak A, Wieligmann K, Bateman OA, Jaenicke R, Slingsby C, J Mol Biol. 2000 Dec 1;304(3):253-7. PMID:11090271

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