1e8e: Difference between revisions

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[[Image:1e8e.gif|left|200px]]
{{Seed}}
[[Image:1e8e.png|left|200px]]


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{{STRUCTURE_1e8e|  PDB=1e8e  |  SCENE=  }}  
{{STRUCTURE_1e8e|  PDB=1e8e  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF METHYLOPHILUS METHYLOTROPHUS CYTOCHROME C''. INSIGHTS INTO THE STRUCTURAL BASIS OF HAEM-LIGAND DETACHMENT'''
===SOLUTION STRUCTURE OF METHYLOPHILUS METHYLOTROPHUS CYTOCHROME C''. INSIGHTS INTO THE STRUCTURAL BASIS OF HAEM-LIGAND DETACHMENT===




==Overview==
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Cytochrome c" from Methylophilus methylotrophus is a monohaem protein with 124 amino acid residues. The iron has two histidine ligands in the oxidised form, one of which detaches and picks up a proton when the protein is reduced. Thus, both forms are paramagnetic. The structure of the oxidised form in solution, determined from NMR data is presented. The family of structures has an average backbone rmsd value of 0.53 A, and a heavy atom rmsd value of 0.95 A, within a target function range of 32 %. This structure is related to class I cytochromes with an additional helix at the N terminus. The haem-binding site occurs in a domain essentially lacking secondary structure motifs and the axial histidinyl residues were found in an unusual near perpendicular orientation. Moreover, a disulfide bridge is present, an uncommon structural feature among c-type cytochromes. The disulfide bridge, linking cysteine residues 96 and 104, forms a loop that confers rigidity and is essential to the detachment of the axial histidine (His95) as demonstrated by chemical disruption of the S-S bond. A route for protonation of the distal histidine involving haem propionate 17 is proposed and discussed in the light of available models for complex membrane proton pumps.
The line below this paragraph, {{ABSTRACT_PUBMED_11327772}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 11327772 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11327772}}


==About this Structure==
==About this Structure==
1E8E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8E OCA].  
1E8E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methylophilus_methylotrophus Methylophilus methylotrophus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8E OCA].  


==Reference==
==Reference==
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[[Category: Paramagnetic]]
[[Category: Paramagnetic]]
[[Category: Redox-bohr effect]]
[[Category: Redox-bohr effect]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:47:25 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:18:56 2008''

Revision as of 21:18, 30 June 2008

File:1e8e.png

Template:STRUCTURE 1e8e

SOLUTION STRUCTURE OF METHYLOPHILUS METHYLOTROPHUS CYTOCHROME C. INSIGHTS INTO THE STRUCTURAL BASIS OF HAEM-LIGAND DETACHMENT

Template:ABSTRACT PUBMED 11327772

About this Structure

1E8E is a Single protein structure of sequence from Methylophilus methylotrophus. Full experimental information is available from OCA.

Reference

Solution structure of Methylophilus methylotrophus cytochrome c": insights into the structural basis of haem-ligand detachment., Brennan L, Turner DL, Fareleira P, Santos H, J Mol Biol. 2001 Apr 27;308(2):353-65. PMID:11327772

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