1e9i: Difference between revisions

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[[Image:1e9i.jpg|left|200px]]
{{Seed}}
[[Image:1e9i.png|left|200px]]


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{{STRUCTURE_1e9i|  PDB=1e9i  |  SCENE=  }}  
{{STRUCTURE_1e9i|  PDB=1e9i  |  SCENE=  }}  


'''ENOLASE FROM E.COLI'''
===ENOLASE FROM E.COLI===




==Overview==
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The crystal structure of Escherichia coli enolase (EC 4.2.1.11, phosphopyruvate hydratase), which is a component of the RNA degradosome, has been determined at 2.5 A. There are four molecules in the asymmetric unit of the C2 cell, and in one of the molecules, flexible loops close onto the active site. This closure mimics the conformation of the substrate-bound intermediate. A comparison of the structure of the E. coli enolase with the eukaryotic enolase structures available (lobster and yeast) indicates a high degree of conservation of the hydrophobic core and the subunit interface of this homodimeric enzyme. The dimer interface is enriched in charged residues compared with other protein homodimers, which may explain our observations from analytical ultracentrifugation that dimerisation is affected by ionic strength. The putative role of enolase in the RNA degradosome is discussed; although it was not possible to ascribe a specific role to it, a structural role is possible.
The line below this paragraph, {{ABSTRACT_PUBMED_11676541}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 11676541 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11676541}}


==About this Structure==
==About this Structure==
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[[Category: Degradosome]]
[[Category: Degradosome]]
[[Category: Lyase]]
[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:22:06 2008''

Revision as of 21:22, 30 June 2008

File:1e9i.png

Template:STRUCTURE 1e9i

ENOLASE FROM E.COLI

Template:ABSTRACT PUBMED 11676541

About this Structure

1E9I is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Escherichia coli RNA degradosome component enolase., Kuhnel K, Luisi BF, J Mol Biol. 2001 Oct 26;313(3):583-92. PMID:11676541

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