1eg0: Difference between revisions

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[[Image:1eg0.gif|left|200px]]
{{Seed}}
[[Image:1eg0.png|left|200px]]


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{{STRUCTURE_1eg0|  PDB=1eg0  |  SCENE=  }}  
{{STRUCTURE_1eg0|  PDB=1eg0  |  SCENE=  }}  


'''FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME'''
===FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME===




==Overview==
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Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A cryo-electron microscopy map of the complex. This map allows identification of RNA helices, peripheral proteins, and intersubunit bridges. Comparison of double-stranded RNA regions and positions of proteins identified in both cryo-EM and X-ray maps indicates good overall agreement but points to rearrangements of ribosomal components required for the subunit association. Fitting of known components of the 50S stalk base region into the map defines the architecture of the GTPase-associated center and reveals a major change in the orientation of the alpha-sarcin-ricin loop. Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits.
The line below this paragraph, {{ABSTRACT_PUBMED_10721991}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_10721991}}


==About this Structure==
==About this Structure==
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[[Category: 70s ribosome]]
[[Category: 70s ribosome]]
[[Category: Low resolution model]]
[[Category: Low resolution model]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:03:07 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:38:21 2008''

Revision as of 21:38, 30 June 2008

File:1eg0.png

Template:STRUCTURE 1eg0

FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME

Template:ABSTRACT PUBMED 10721991

About this Structure

1EG0 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Solution structure of the E. coli 70S ribosome at 11.5 A resolution., Gabashvili IS, Agrawal RK, Spahn CM, Grassucci RA, Svergun DI, Frank J, Penczek P, Cell. 2000 Mar 3;100(5):537-49. PMID:10721991

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