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| {{STRUCTURE_1enh| PDB=1enh | SCENE= }} | | {{STRUCTURE_1enh| PDB=1enh | SCENE= }} |
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| '''STRUCTURAL STUDIES OF THE ENGRAILED HOMEODOMAIN'''
| | ===STRUCTURAL STUDIES OF THE ENGRAILED HOMEODOMAIN=== |
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| ==Overview==
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| The structure of the Drosophila engrailed homeodomain has been solved by molecular replacement and refined to an R-factor of 19.7% at a resolution of 2.1 A. This structure offers a high-resolution view of an important family of DNA-binding proteins and allows comparison to the structure of the same protein bound to DNA. The most significant difference between the current structure and that of the 2.8-A engrailed-DNA complex is the close packing of an extended strand against the rest of the protein in the unbound protein. Structural features of the protein not previously noted include a "herringbone" packing of 4 aromatic residues in the core of the protein and an extensive network of salt bridges that covers much of the helix 1-helix 2 surface. Other features that may play a role in stabilizing the native state include the interaction of buried carbonyl oxygen atoms with the edge of Phe 49 and a bias toward statistically preferred side-chain dihedral angles. There is substantial disorder at both ends of the 61 amino acid protein. A 51-amino acid variant of engrailed (residues 6-56) was synthesized and shown by CD and thermal denaturation studies to be structurally and thermodynamically similar to the full-length domain. | | The line below this paragraph, {{ABSTRACT_PUBMED_7849596}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 7849596 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_7849596}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Pabo, C O.]] | | [[Category: Pabo, C O.]] |
| [[Category: Dna-binding protein]] | | [[Category: Dna-binding protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:18:47 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:04:41 2008'' |
